The Presence of a Thyrotropin-Releasing Hormone-Like Factor in the Grass Shrimp (Black Tiger Prawn, Penaeus monodon)

草蝦體內存在甲釋素類似物之研究

謝易修(Yih-Shou Hsieh) ； 范夢樵(Mon-Chou Fann) ； 萬家茂(W. Chia-Mo Wan) ； 王錫崗(Paulus S. Wang)

《The Chinese Journal of Physiology》 33卷2期 (1990/06) Pp. 179-190

摘要

本研究以放射免疫法測得草蝦肝胰臟之鹽酸或甲醇萃取物中，含有類似甲釋素之免疫活性。另以甲釋素刺激大鼠離體腦下腺甲促素釋放之作用，測得甲釋素之生物活性。唯草蝦肝胰臟之類似甲釋素之物質經進一步實驗，發現其分子量較甲釋素大，其他特性，例如無法以甲釋素抗體中和，亦不會被正常大鼠血清破壞，均與甲釋素不同。

並列摘要

Immunoreactive (TRH) has been detected in acid and methanol extracts of hepatopancreas, in grass shrimp (black tiger prawn, Penaeus monodon).The evidence provided by the stimulation of thyrotropin (TSH) release from perfused rat anterior pituitary glands in vitro following the application of hepatopancreas extracts (SHE) reflected the presence of TRH-like substance in the shrimp. However, the molecular weight of the TRH-like substance estimated by gel filtration in SHE was greater than that of the synthetic TRH. Furthermore, other differences are also noted, e.g. TRH-like activity in SHE could not be completely neutralized by anti-TRH antiserum nor degraded in normal rat serum.