Antiplatelet Activity of Caffeic Acid Phenethyl Ester is Mediated through a Cyclic GMP-Dependent Pathway in Human Platelets

Tyng-Guey Chen ； Jie-Jen Lee ； Kuang-Hung Lin ； Chia-Hung Shen ； Duen-Suey Chou ； Joen-Rong Sheu

《The Chinese Journal of Physiology》 50卷3期 (2007/06) Pp. 121-126

並列摘要

The aim of this study was to examine the inhibitory mechanisms of caffeic acid phenethyl ester (CAPE), which is derived from the propolis of honeybee, in platelet activation. In this study, CAPE (15 and 25 μM) markedly inhibited platelet aggregation stimulated by collagen (2 μg/ml). CAPE (15 and 25 μM) increased cyclic GMP level, and cyclic GMP-induced vasodilator-stimulated phosphoprotein (VASP) Ser^157 phosphorylation, but did not increase cyclic AMP in washed human platelets. Rapid phosphorylation of a platelet protein of Mw. 47,000 (P47), a marker of protein kinase C activation, was triggered by phorbol-12, 13-dibutyrate (150 nM). This phosphorylation was markedly inhibited by CAPE (15 and 25 μM). The present study reports a novel and potent antiplatelet agent, CAPE, which involved in the following inhibitory pathways: CAPE increases cyclic GMP/VASP Ser^157 phosphorylation, and subsequently inhibits protein kinase C activity, resulting in inhibition of P47 phosphorylation, which ultimately inhibits platelet aggregation. These results strongly indicate that CAPE appears to represent a novel and potent antiplatelet agent for treatment of arterial thromboembolism.

並列關鍵字

caffeic acid phenethyl ester ； cyclic GMP ； vasodilator-stimulated phosphoprotein ； protein kinase C

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