- 期刊
Attenuation of Pulmonary Arterial Smooth Muscle Cell Proliferation Following Hypoxic Pulmonary Hypertension by the Na(superscript +)/H(superscript +) Exchange Inhibitor Amiloride
並列摘要
Chronic hypoxia results in pulmonary hypertension. To investigate the role of Na(superscript+)/H(superscript+) exchange in this process, determined the effect of amiloride, a Na(superscript+)H(superscript+) exchange inhibitor, on hypoxic pulmonary hypertension and pulmonary arterial smooth muscle cell proliferation, both in vivo and in vitro. Sprague-Dawley rats ere placed either in a hypobaric, hypoxic chamber (10.5% O2) or under normal 21% O2 atmosphere for 8 h each day for 3 weeks. Rats under hypoxic conditions received 1,3, or 10 mg/kg/d amiloride or the vehicle alone. Hematologic indices, including red blood cells, hemoglobin, hematocrit and mean corpuscular hemoglobin increased in hypoxic rats, but these changes ere prevented by treatment with amiloride. In the hypoxic rats, the right ventricular systolic pressure and right ventricular hypertension index (weight ratio of right ventricular to left and septum together) were increased by 88% and 129%, respectively. Arteriolar all thickness and area in the hypoxia-treated animals increased 3-and 2-fold, respectively, over normoxic controls; the increase in each of these indices as attenuated by amiloride in a dose-dependent manner. In cultured pulmonary arterial smooth muscle cells, hypoxia greatly increased cellular proliferation, and this similarly shoed a dose-dependent attenuation in the presence of amiloride. Amiloride did not affect blood pressure in vivo or cause cell damage in vitro. These data suggest that the Na(superscript +)/H(superscript +) exchange inhibitor amiloride ma represent an effective adjunctive therapy in pulmonary hypertension induced b chronic hypoxia.
參考文獻
延伸閱讀
- HongueroMartínez, A. F., Obrer, A. A., Almánzar, S. F., Atance, P. L., & Jorge, R. G. (2014). Analysis of Expression of Vascular Endothelial Growth Factor A and Hypoxia Inducible Factor-1alpha in Patients Operated on Stage I Non-Small-Cell Lung Cancer. Lung Cancer International, (2014), c1-6. https://doi.org/10.1155/2014/810786
- 張齡如（2005）。The Alteration of Tumor Cell Induced Angiogenesis and its Surface Adhesion Molecules Expression Caused by Hypoxia and their Effects〔碩士論文，國立臺灣大學〕。華藝線上圖書館。https://doi.org/10.6342/NTU.2005.02415
- Chen, G. S., Wu, C. H., Liau, C. C., Hsu, C. C., Liu, J. Y., Wu, G. J., Chou, C. C., & Loh, S. H. (2015). Physiological and Pharmacological Characterization of Transmembrane Acid Extruders in Cultured Human Umbilical Artery Smooth Muscle Cells. Journal of Medical Sciences, 35(5), 208-217. https://doi.org/10.4103/1011-4564.167775
- Hsieh, P. L. (2010). The inhibitory effects and action mechanism of compound 5185 on proliferation of vascular smooth muscle cells and neointima formation in rat carotid arteries [master's thesis, National Taiwan University]. Airiti Library. https://doi.org/10.6342/NTU.2010.10208
- 荘哲舜、張玉亭、蔡勇男、秦作威、廖美華、溫小娟（2008）。Expression of Hypoxia Inducible Factor (HIF-1α), Matrix Metalloproteinase-2(MMP-2) and Heat Shock Protein 70 (HSP70) in Lung after Pre-hypoxic and Post-hypoxic Stroke Induced by Middle Cerebral Artery。加馬，(39)，23-30。https://doi.org/10.30173/JG.200806.0005