Resveratrol Protects Left Ventricle by Increasing Adenylate Kinase 1 and Isocitrate Dehydrogenase Activities in Rats with Myocardial Infarction

Jeng-Feng Lin ； Semon Wu ； Shiang-Suo Huang ； Bo-Yi Lu ； Su-Man Lin ； Shen-Kou Tsai

《The Chinese Journal of Physiology》 54卷6期 (2011/12) Pp. 406-412

https://doi.org/10.4077/CJP.2011.AMM097

並列摘要

Our prior study had shown that resveratrol was a potent cardioprotective agent in rats with myocardial infarction (MI). In this study, we further evaluated the mechanism of cardioprotection of resveratrol by proteomic analysis. After permanent ligation of the left anterior descending artery under isoflurane anesthesia, surviving rats were randomly allocated to three groups and treated with resveratrol at 1 mg/kg/day (MI/R group), or vehicles (sham group and MI group) once daily for four weeks. In proteomic analysis, the MI group showed decreased expression of adenylate kinase 1 (AK1) and mitochondrial NADP(superscript +)-dependent isocitrate dehydrogenase (IDPm) after MI compared with the sham group. These variations were reversed by resveratrol in the MI/R group. Validation with Western blot and immunohistochemical analyses showed similar trends in protein expression profiling. Our studies suggest that the beneficial effects of resveratrol on ventricular modeling may be due to increased expression of AK1 and IDPm, which have been known to increase myocardial energetic efficiency and reduce reactive oxygen species-mediated damage, respectively.

並列關鍵字

resveratrol ； adenylate kinase 1 ； isocitrate dehydrogenase ； myocardial infarction

參考文獻

Baur, J.A.,Pearson, K.J.,Price, N.L.,Jamieson, H.A.,Lerin, C.,Kalra, A.,Prabhu, V.V.,Allard, J.S.,Lopez-Lluch, G.,Lewis, K.,Pistell, P.J.,Poosala, S.,Becker, K.G.,Boss, O.,Gwinn, D.,Wang, M.,Ramaswamy, S.,Fishbein, K.W.,Spencer, R.G.,Lakatta, E.G.,Le Couteur, D.,Shaw, R.J.,Navas, P.,Puigserver, P.,Ingram, D.K.,de Cabo, R.,Sinclair, D.A.(2006).Resveratrol improves health and survival of mice on a high-calorie diet.Nature.444,337-342.

Braunwald, E.(2008).Biomarkers in heart failure.N. Engl. J. Med..358,2148-2159.

Chen, C.J.,Yu, W.,Fu, Y.C.,Wang, X.,Li, J.L.,Wang, W.(2009).Resveratrol protects cardiomyocytes from hypoxia-induced apoptosis through the SIRT1-FoxO1 pathway.Biochem. Biophys. Res. Commun..378,389-393.

Chen, Y.R.,Yi, F.F.,Li, X.Y.,Wang, C.Y.,Chen, L.,Yang, X.C.,Su, P.X.,Cai, J.R.(2008).Resveratrol attenuates ventricular arrhythmias and improves the long-term survival in rats with myocardial infarction.Cardiovasc. Drugs Ther..22,479-485.

Dargie, H.J.(2001).Effect of carvedilol on outcome after myocardial infarction in patients with left-ventricular dysfunction: the CAPRICORN randomized trial.Lancet.357,1385-1390.