Anti-Inflammatory Effects of Probiotic Lactobacillus paracasi on Ventricles of BALB/C Mice Treated with Ovalbumin

Hsueh-Fang Wang ； Chien-Yu Tseng ； Mu-Hsin Chang ； James A Lin ； Fu-Jen Tsai ； Chang-Hai Tsai ； Ying-Chen Lu ； Chao-Hung Lai ； Chih-Yang Huang ； Cheng-Chih Tsai

《The Chinese Journal of Physiology》 55卷1期 (2012/02) Pp. 37-46

https://doi.org/10.4077/CJP.2012.AMM107

並列摘要

Lactic acid bacteria (LAB) are microorganisms that benefit animals with allergic diseases and intestinal disorders such as inflammatory bowel disease. We propose that LAB can prevent cardiomyocytes inflammation and apoptosis in BALB/c mice using an ovalbumin (OVA)-induced allergy. Thirty-nine male BALB/c mice were divided into five groups: normal control, allergy control and three allergy groups each treated with Kefir I (Kefir I), Kefir II (Kefir II) or GM080 products (GM080). The myocardial architecture and apoptotic molecules in the excised left ventricle from these mice were investigated and post-treatment effects were evaluated. The inflammatory pathway, including toll-like receptor 4 (TLR4), phospholate-Jun-N-terminal kinase (p-JNK), JNK1/2 and tumor necrosis factor-alpha (TNF-α) and the mitochondria-dependent apoptosis phospholate-p38 (p-p38), Bcl-2 associated agonist of cell death (Bad), Bcl-2 associated X (Bax) and activated caspase 3, were found to be significantly increased in the hearts of allergy mice. The expression of phospholate-nuclear factor-κB (p-NFκB), TNF-α, p-p38 and Bad protein products were reduced or retarded in the Kefir I- or II-treated allergy group. The GM080-treated allergy group exhibited significantly lower p-JNK, JNK1/2, phospholate-Ikappa B (p-IκB), Bax and Bad protein products than the Kefir I and Kefir II allergy groups. These results indicate that LAB can reduce inflammation and prevent apoptosis of cardiomyocytes in the heart of OVA-induced allergy mice.

並列關鍵字

allergy ； inflammation ； apoptosis ； lactic acid bacteria ； ovalbumin

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