透過您的圖書館登入
IP:44.192.247.185
  • 期刊

High-Frequency Stimulation of the Subthalamic Nucleus Activates Motor Cortex Pyramidal Tract Neurons by a Process Involving Local Glutamate, GABA and Dopamine Receptors in Hemi-Parkinsonian Rats

摘要


Deep brain stimulation (DBS) is widely used to treat advanced Parkinson’s disease (PD). Here, we investigated how DBS applied on the subthalamic nucleus (STN) influenced the neural activity in the motor cortex. Rats, which had the midbrain dopaminergic neurons partially depleted unilaterally, called the hemi-Parkinsonian rats, were used as a study model. c-Fos expression in the neurons was used as an indicator of neural activity. Application of high-frequency stimulation (HFS) upon the STN was used to mimic the DBS treatment. The motor cortices in the two hemispheres of hemi-Parkinsonian rats were found to contain unequal densities of c-Fos-positive (Fos^+) cells, and STN-HFS rectified this bilateral imbalance. In addition, STN-HFS led to the intense c-Fos expression in a group of motor cortical neurons which exhibited biochemical and anatomical characteristics resembling those of the pyramidal tract (PT) neurons sending efferent projections to the STN. The number of PT neurons expressing high levels of c-Fos was significantly reduced by local application of the antagonists of non-N-methyl-D-aspartate (non-NMDA) glutamate receptors, gamma-aminobutyric acid A (GABA_A) receptors and dopamine receptors in the upper layers of the motor cortex. The results indicate that the coincident activations of synapses and dopamine receptors in the motor cortex during STN-HFS trigger the intense expression of c-Fos of the PT neurons. The implications of the results on the cellular mechanism underlying the therapeutic effects of STN-DBS on the movement disorders of PD are also discussed.

參考文獻


Beaulieu, J.M.,Espinoza, S.,Gainetdinov, R.R.(2015).Dopamine receptors - IUPHAR Review 13.Brit. J. Pharmacol..172,1-23.
Benabid, A.L.,Pollak, P.,Louveau, A.,Henry, S.,de Rougemont, J.(1987).Combined (thalamotomy and stimulation) stereotactic surgery of the VIM thalamic nucleus for bilateral Parkinson disease.Appl. Neurophysiol..50,344-346.
Benavides-Piccione, R.,DeFelipe, J.(2007).Distribution of neurons expressing tyrosine hydroxylase in the human cerebral cortex.J. Anat..11,212-222.
Blumenfeld, Z.,Bronte-Stewart, H.(2015).High frequency deep brain stimulation and neural rhythms in Parkinson's disease.Neuropsychol. Rev..25,384-397.
Boix, J.,Padel, T.,Paul, G.(2015).A partial lesion model of Parkinson's disease in mice--characterization of a 6-OHDA-induced medial forebrain bundle lesion.Behav. Brain Res..284,196-206.

延伸閱讀