Cultivation of Absidia coerulea for Chitosan Production in a Modified Airlift Reactor

本研究採用有兩個同心內網管的改良氣舉式發酵槽培養Absidia coreulea菌種，發酵生產幾丁聚醣。在此發酵過程中微生物對溶氧的需求很高，且對剪應力的強度敏感。改良後的氣舉式發酵槽有足夠的氣液質傳能力和較低剪應力，提供了一個良好的菌體生長條件。在此發酵槽生產的幾丁聚醣產量比傳統的氣泡塔式及600轉的攪拌式發酵槽的產量高兩倍，比300轉的攪拌式產量高55％。利用所提之反應器，分子量及去乙醯度分別為1050kDa及92.5%。此值比攪拌槽中所得到之值高出很多。在600rpm之攪拌槽，分子量和去乙醯度分別為710kDa及85.2%。在300rpm下，其值為820kDa及88.1%。採用氣泡塔，分子量及去乙醯度分別為950kDa及92.2%。

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並列摘要

Cultivation of Absidia coerulea for chitosan production was carried out in a modified airlift reactor with two concentric wire mesh draft tubes. The oxygen requirement for the cultivation was high and the microorganisms were sensitive to shear stress. The modified reactor which had high oxygen transfer capability provided a suitable environment for the cultivation. Production of chitosan in the modified reactor was more than two times higher than those in both the conventional bubble column and the stirred tank fermenter with impeller speed of 600 rpm and 55% higher than that in the stirred tank fermenter with the impeller speed of 300 rpm. By using the proposed reactor, the molecular weight and degree of deacetylation of chitosan at the end of 65 hr cultivation were 1050 kDa and 92.5 %, respectively, which were much higher than those using the stirred tank fermenter. The values were 710 kDa and 85.2 % for the stirred tank fermenter with impeller speed of 600 rpm; 820 kDa and 88.1 % for the stirred tank fermenter with impeller speed of 300 rpm. By using the bubble column, the molecular weight and degree of deacetylation were 950 kDa and 92.2%, respectively.

並列關鍵字

Chitosan ； Absidia coerulea ； Modified Airlift Reactor

國際替代計量

Cultivation of Absidia coerulea for Chitosan Production in a Modified Airlift Reactor

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