水稻不同次位分蘖、穗位及枝梗間花葯培養癒傷組織之形成

The Callus-forming Ability of Rice Anthers Originated from Tillers of Different Orders and from Spikelets of Different Positions and Branches on the Panicles

陳駿季(J. J. Chen) ； 蔡新聲(H. S. Tsay)

《中華農業研究》 33卷4期 (1984/12) Pp. 354-362

https://doi.org/10.29951/JARC.198412.0002

摘要

以臺農67號與臺南5號兩水稻栽培品種爲材料，利用兩種不同取樣方式比較不同次位分蘖，不同穗位及不同梗梗間花莉癒傷組織的形成能力。第一種取樣方式是在同一株水稻中，從主桿到第三次分蘖，隨著其發育而依序取樣，以此種取樣方式，主桿與分茶間之花滿培養癒傷組織形成能力極爲一致；第二種取樣方式是每一株水稻只取一特定次位分藥，其餘分蘖則轉較為是保韶在植株上，此種取樣的結果，主桿與一、二次分aynd花莉癒傷組織的形成能力仍大致相同，三次分蘖則較爲低下。第二種取樣方式同時顯示對於開花末期接種的花藥癒傷組織形成能力有不利的影響。
穗位及枝梗間花滿癒傷組織之形成在兩種取樣方式下都沒有差異。取樣方式的不同造成第三次分蘖及較晚之二次分蘖花茄癒傷組織形成能力差異的原因，本文亦有所討論。
本試驗同時顯示花葯培養的成效受母體植株生長環境所左右。三次不同接種月份中，以1981年5月份所接種的花葯,其癒傷組織形成能力最高。

並列摘要

The objective of this study was to compare the callus-forming ability of anthers originated from rice tillers of different orders; pikelets at different position and branches.
Two methods of sampling panicles for anthers culture were employed. The first method was to collect panicles at the appropriate developmental stage from rice tillers of different orders within the same plant. The second method was to harvest panicles from tillers of a definite order from individual plant, vegetative organs were remained in both sampling methods.
No difference in callus-orming ability of anthers was found amoung tillers of different orders when the first method was used. By using the second method, it was found that the callus-orming ability of anthers from the tertiary tillers was lower than that from main culm and primary and secondary tillers.
Tillers developed earlier bear anthers of higher callus-orming ability than those developed later but of the same order.
No difference in callus-forming ability of anthers was found among spikelets from different position or branches of panicles. The reason of low callus-orming ability of anthers from tertiary and later developed secondary tillers was discussed.
Sampling made in May 1981 showed the highest callus-forming ability which showed that the efficiency of anthers culture was influenced by the enviroment in which the donor plants were cultured.