An inoculation method for screening resistance to Pythium stalk rot (Pythium aphanidermatum) in corn cultivars was established in this study. Three seeds of each corn cultivar or line were sowed on a six-inch-pot and grown in a greenhouse at temperature between 24 and 36 C. After 40~45 days incubation until corn plants reached about 100 cm in height, they were used for inoculation. The artificial infested soil containing 30 oospores per gram of air-dry soil was saturated with water and smeared on the basal stalk (0~10 cm) of the tested plants. The inoculated site was wrapped with a piece of aluminum foil to keep the inoculum from drying out. The inoculated plants were incubated in the greenhouse with temperature at 25 to 35 C and relative humidity at 90% or more for three days. Thereafter, the environment was recovered to normal conditions for another three days. In general, cultivars (lines) susceptible to the fungus would readily lodge, whereas, those resistant ones stayed as healthy as non-inoculated plants. By this method, about 42% of 1100 tested corn cultivars (lines) were screened as resistant to Pythium stalk rot in our study.
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