並列摘要
The purpose of this experiment was to develop effective in vitro method to mass-propagate ginger flower in a short period of time as required for commercial cultivation. Shoot tips (1 mm in size) were cultured on MS medium containing 2 mg/l of 6-benzyladenine, 0.05 mg/l of NAA, 40 mg/l of adenine sulfate, 170 mg/l of NaH2PO4•H2O and 3% of sucrose for 1 month. The adventitious buds produced from these cultures were used to evaluate the effect of topping and the composition of culture medium on the production of secondary adventitious buds. The results are summarized as follows 1. Full strength of MS basic salts supplemented with 4 mg/l of 6-benzyladenine was able to produce an average of 4.78 new shoots per cultured adventitious bud within 6 weeks. Rooting rate was as high as 100% after 4 weeks in culture. 2. Propagation rate was faster for liquid medium than for agar medium. Number of shoots produced per cultured adventitious bud was higher from untopping treatment than from topping treatment, and rooting ratio of the newly produced shoots was as high as 100% after 2 weeks in culture. According to the propagation method developed in this study, 8×10^5 seedlings could be produced from one shoot tip explant in a year.
延伸閱讀
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