彩虹鳥蕉組織培養之研究Ⅲ. Cefotaxime和植物生長調節劑對莖頂培植體生育之影響

Studies on Tissue Culture of Heliconia psittacorum cv. Rhizomatosa Ⅲ. Effect of Cefotaxime and Plant Growth Regulators on Growth of Shoot Tip Explants in Vitro

蕭翌柱(Yih-Juh Shiau) ； 王才義(Tsai-Yih Wang) ； 蔡新聲(Hsin-Sheng Tsay)

《中華農業研究》 48卷4期 (1999/12) Pp. 42-51

https://doi.org/10.29951/JARC.199912.0004

摘要

本研究目的在探討cefotaxime抗生素預處理彩虹鳥蕉(Heliconia psittacorum cv. Phizomatosa)莖頂培植體對降低細菌污染及生育的影響，並探討以組織培養大量繁殖種苗的可行性。結果顯示，莖頂培植體接種於含MS基本鹽類並添加500mg/l cefotaxime的夜體培養基預培養3天後，再繼代培養於固體培養基，對降低細菌污染有顯著的效果，且不會對莖頂組織造成傷害。培植體培養於MS基本鹽類並添加4-8mg/l BA和0.5mg/l NAA的培養基45天後，對促進新莖節萌發效果良好；而接種於含0.01-0.5mg/l TDZ固體培養之莖頂培植體，也顯示有促進莖節快速生長的作用。另外，本試驗也探討不同的培養方式對彩虹鳥蕉新芽莖頂生育之影響，並獲得初步成果。

關鍵字

Cefotaxime ； Thidiazuron ； 彩虹鳥蕉 ； 離體培養

並列摘要

This paper describes a method of in vitro propagation of Heliconia psittacorum cv. Rhizomatosa by using terminal shoot tip explants. Various concentrations (25,50,100 and 500 mg/l) of cefotaxime were used in liquid Murashige and Skoog (MS) basal medium to control contamination. Lower rate of contamination was observed in shoot tip explants after pretreatment of explants in liquid MS medium supplemented with 500 mg/l cefotaxime for 3 days. Well developed shoots were successfully obtained after 45 days of culture of shoot tip explants on MS medium supplemented with 4-8 mg/l Benzylaminopurine (BA) and 0.5 mg/l α-Naphthaleneacetic acid (NAA). Addition of 0.01-0.5 mg/l Thidiazuron (TDZ) in the medium was found to be beneficial for rapid growth of shoot tip explants. The effect of various culture methods on growth of shoot tip explants was also studied.

並列關鍵字

Cefotaxime ； Thidiazuron ； Heliconia psittacorum cv. Rhizomatosa ； In vitro culture