以逢機增殖多型性DNA (RAPD)進行火鶴花栽培種鑑定與遺傳歧異性之分析

Randomly Amplified Polymorphic DNA (RAPD) Markers for Identification and Genetic Diversity Analysis of Anthurium Cultivars

王昭月(Jau-Yueh Wang) ； 莊耿彰(Keng-Chang Chuang) ； 范明仁(Ming-Jen Fan)

《中華農業研究》 48卷4期 (1999/12) Pp. 52-63

https://doi.org/10.29951/JARC.199912.0005

摘要

本研究以農業試驗所『火鶴花育種計畫』中收集的15個栽培種為材料，探討利用逢機增殖多型性DNA (randomly amplified polymorphic DNA; RAPD)分子技術作為品種鑑定及遺傳歧異性分析。試驗結果顯示，自8條含有10個核甘酸的逢機引子經聚合酵素連鎖反應(polymerase chain reaction; PCR)產物中，有24個特定的DNA多型性標誌片段，可用以鑑別出14個栽培種。進一步以此8個多型性表現高的引子，將其PCR反應產物160個RAPD片段，合併進行UPGMA (unweighted Pair-group mean arithmetic)群集分析，結果獲得三個主要群組。第一群包含8個栽培種，其佛燄苞顏色（花色）主要為紅色系。第二群為6個栽培種組成，主要為源自夏威夷育成之品種，而第一、二兩群14個栽培種均屬Anthurium andraeanum。第三群則僅有一個獨立的栽培種”southern Blush”，為A. andraeanum X A. amnicola之種間雜交種。試驗結果顯示，利用RAPD兼具有鑑別火鶴花栽培種之能力，亦可用以估測品種間基因型之遺傳相似性，此對於未來藉以選拔遺傳歧異高的雜交親本及新雜交組合的獲得，將有所助益。整體而言，RAPD分析技術對於加速火鶴花育種效率，應具有實用價值。

關鍵字

火鶴花 ； 育種 ； 逢機增殖多型性DNA ； 鑑定 ； 遺傳歧異性

並列摘要

Fifteen Anthurium cultivars collected for the breeding program of Taiwan Agriculture Research Institute were evaluated by randomly amplified polymorphic DNA(RAPD) technique. Objectives were to assess genetic diversity within the cultivars and survey the identification markers. Twenty-four DNA fragments derived from eight primers are polymorphic and can be used to distinguish the fourteen cultivars. A dendrogram generated by UPGMA(Un-weighted Pair-group Mean Arithmetic) cluster analysis from 160 RAPD fragments that all amplified by eight primers, divided the fifteen cultivars onto three main groups. The first group comprised eight cultivars, all with red spathe. The second group comprised six cultivars that mainly original derived from the breeder of Hawaii. The fourteen cultivars within these two groups, belonging to Anthurium andraeanum, while only with one culivar ”Southern Blush” in the third group, is a hybrid from A. andraeanum X A. amnicola. The results suggest that RAPD markers are useful in current breeding program of Anthurium, and allowing the identification within different cultivars as well as the estimation the genetic similarity among genotypes, which will be valuable in selecting the best parents and to obtain new combinations.

並列關鍵字

Anthurium ； Breeding ； Random Amplified Polymorphic DNA RAPD ； Identification ； Genetic diversity