An 8-week feeding trial was conducted to establish the effect of dietary selenium (Se) on non-specific immune responses of grouper, Epinephelus malabaricus. Selenomethionine was added to the basal diet at 0, 0.5, 1, 2, 3, 5 and 10 mg Se/kg diet, providing 0.21, 0.77, 1.38, 2.02, 2.70, 4.00 and 7.91 mg Se/kg diet, respectively. Each diet was fed to triplicate groups of fish (mean initial weight: 12.20±0.14 g) in a closed, recirculating rearing system. Leukocyte respiratory burst [superoxide anion O2(superscript -) production ratio] activity and plasma immunoglobulin concentration were higher (P<0.05) in fish fed diets with 077-2.02 mg Se/kg than that in fish fed diets with≥2.70 mg Se/kg diet and the unsupplemented control diet. Fish fed diets with 0.77-2.70 mg Se/kg diet had higher plasma lysozyme activity than fish fed diets with≥4.00 mg Se/kg diet and the control diet. Hepatic vitamin E concentration was highest in fish fed diets with≥1.38 mg Se/kg diet, followed by fish fed diet with 0.77 mg Se/kg diet, and lowest in fish fed the control diet. Hepatic Se concentration was highest in fish fed diet with 7.91 mg Se/kg, followed by 5 mg Se/kg, then 2 mg Se/kg, 1 mg Se/kg diet, and lowest in fish fed the control diet. Hepatic thiobarbituric acid reactive substances (TBARS) value was highest in fish fed the control diet, followed by fish fed diet with 0.77 mg Se/kg diet, and lowest in fish fed diets with≥1.38 mg Se/kg diet. The results suggest that juvenile grouper fed diets with 0.8-2 mg Se/kg diet enhanced their non-specific immune responses.
An 8-week feeding trial was conducted to establish the effect of dietary selenium (Se) on non-specific immune responses of grouper, Epinephelus malabaricus. Selenomethionine was added to the basal diet at 0, 0.5, 1, 2, 3, 5 and 10 mg Se/kg diet, providing 0.21, 0.77, 1.38, 2.02, 2.70, 4.00 and 7.91 mg Se/kg diet, respectively. Each diet was fed to triplicate groups of fish (mean initial weight: 12.20±0.14 g) in a closed, recirculating rearing system. Leukocyte respiratory burst [superoxide anion O2(superscript -) production ratio] activity and plasma immunoglobulin concentration were higher (P<0.05) in fish fed diets with 077-2.02 mg Se/kg than that in fish fed diets with≥2.70 mg Se/kg diet and the unsupplemented control diet. Fish fed diets with 0.77-2.70 mg Se/kg diet had higher plasma lysozyme activity than fish fed diets with≥4.00 mg Se/kg diet and the control diet. Hepatic vitamin E concentration was highest in fish fed diets with≥1.38 mg Se/kg diet, followed by fish fed diet with 0.77 mg Se/kg diet, and lowest in fish fed the control diet. Hepatic Se concentration was highest in fish fed diet with 7.91 mg Se/kg, followed by 5 mg Se/kg, then 2 mg Se/kg, 1 mg Se/kg diet, and lowest in fish fed the control diet. Hepatic thiobarbituric acid reactive substances (TBARS) value was highest in fish fed the control diet, followed by fish fed diet with 0.77 mg Se/kg diet, and lowest in fish fed diets with≥1.38 mg Se/kg diet. The results suggest that juvenile grouper fed diets with 0.8-2 mg Se/kg diet enhanced their non-specific immune responses.