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An Immunological Study of the Red Drum (Sciaenops ocellatus, Linnaeus) -Specific 80 kDa Protein in Taiwan Fishes

紅鼓魚的免疫學研究-台灣魚種中特有80 kDa蛋白質之探討

摘要


美國佛羅里達州的兩位科學家於1995年認定80 kDa蛋白質為紅鼓魚專有,可用於判定新放流幼魚被捕食時之分子標記。在本實驗中,我們驗證了此種蛋白質在台灣魚群中的特異性。採用所生產之80 kDa蛋白質特異之抗兔血清,以「西式轉漬法」測試了在台灣海域所收集的三十五種不同的魚種。結果發現除了紅鼓魚身上有此蛋白質外,並無其他測試之魚種具有此蛋白質;再度確認了此種蛋白質為紅鼓魚專屬之蛋白質。因此,將來可應用其作為紅鼓魚之生物標記,以定位無法辨認之被捕食的紅鼓魚。

並列摘要


Scientists in Florida, USA, had identified a red drum (Sciaenops ocellatus)-specific 80 kDa protein, which was applied in determining the predation mortality of newly released fish. Here, we verified the specificity of this protein in fish community of Taiwan. Using a specific rabbit antiserum, produced against the 80 kDa red drum protein, we tested 35 different species of fish collected around Taiwan for this protein by Western blots. Except in red drum, this specific 80 kDa protein was not detected in all other fish species tested. Our results confirm the specificity of the 80 kDa protein to red drum, indicating that this protein can be applied as a biomarker to locate otherwise unidentifiable red drum fish in Taiwan.

並列關鍵字

Red drum 80 kDa protein Specificity Fish of Taiwan Biomarker

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