Identification of Toxin and Species of Causative Fish Roe Implicated into a Food Poisoning Incident

魚卵引起之食物中毒事件之毒性及魚種的鑑定

吳雅蓉(Ya-Jung Wu) ； 任曉晶(Hsiao-Chin Jen) ； 鄧昭芳(Jou-Fang Deng) ； 黃登福(Deng-Fwu Hwang)

《臺灣水產學會刊》 35卷4期 (2008/12) Pp. 359-367

https://doi.org/10.29822/JFST.200812.0006

摘要

2008年3月，台灣發生一起食用河魨魚卵中毒之事件，患者共有3人。患者自行購買市售之魚卵，食入後出現嘔吐、舌頭、口唇及手腳麻痺等症狀。症狀與河魨毒中毒者極為相似，因此依河魨毒毒性分析及粒線體DNA基因技術鑑定魚卵。由生物毒性檢定法測定魚卵之毒性，經實驗結果證實每克魚卵中含有425±80個老鼠單位(MU)。以高效能液相層析法及液相層析質譜儀分析鑑定其毒成分，得知含有河魨毒(Tetrodotoxin)。利用粒線體基因cytochrome b部分序列及PCR-RFLP分析法應用於鑑定未知魚種之魚卵，結果得知導致中毒的河魨魚卵為栗色河魨(Lagocephalus lunaris)。綜合上述，本次食物中毒之毒成分係為河魨毒所致，中毒魚卵之河魨則為有毒種L. lunaris。

關鍵字

食物中毒 ； 栗色河魨 ； 河魨毒 ； 液相層析質譜儀 ； 聚合酶鏈鎖反應－限制酶限制片段長度多型性

並列摘要

The food poisoning incident due to ingesting fish roe occurred in Taiwan in March 2008. A total of 3 victims were reported. Symptoms featured paralysis, coma, nausea, vomiting and ataxia. Using the cytochrome b gene sequence and PCR-RFLP method identified the fish species of the roe. The results indicated that species of causative fish roe was Lagocephalus lunaris. Toxicity of causative fish roe was 425 ± 80 mouse units (MU) by a tetrodotoxin (TTX) bioassay. The toxin was partially purified from the acidic methanol extract of the roe by using a C18 solid phase extraction column. The eluate was then filtered through a 3,000 dalton cut-off ultrafree microcentrifuge filter. Analyses of high performance liquid chromatography (HPLC) and liquid chromatography/mass spectrometry (LC/MS) identified the purified toxin mainly containing TTX. It is concluded that the causative agent of the above food poisoning was TTX and the roe species was a toxic puffer L. lunaris. This food poisoning is a case of puffer intoxication.

並列關鍵字

food poisoning ； Lagocephalus lunaris ； tetrodotoxin ； LC/MS ； PCR-RFLP