- 期刊
- OpenAccess
White Shrimp Litopenaeus vannamei Immersed in Seawater Containing Ulva lactuca Powder, and its Filtrate, Residue, and Extract Showed Increased Immunity and Resistance Against Vibrio alginolyticus
白蝦接受石蓴粉末、及其濾液，殘留物，萃取物增加免疫力及溶藻弧菌抵抗
摘要
The effects of Ulva lactuca powder, its filtrate, residue, and extract on the immune response of white shrimp Litopenaeus vannamei were studied in vitro and in vivo. Shrimp hemocytes incubated in the powder, filtrate, residue, and extract significantly increased their phenoloxidase (PO) activity and generation of superoxide anion. The hyaline cell (HC), granular cell (including semi-granular cell, GC), total hemocyte count (THC), phenoloxidase (PO) activity, and respiratory burst were examined after the shrimp were immersed in seawater containing the powder, its filtrate, and residue at 500 mg/l, and extract at 200 mg/l for 1, 3, and 5 h. These immune parameters were significantly higher in shrimp receiving the powder, filtrate, residue, and extract than in control shrimp after 3-5 h. In another experiment, shrimp immersed in seawater containing the powder, filtrate, residue, and extract after 3 h were challenged with Vibrio alginolyticus at 1 × 10^6 colony-forming unit (cfu) per shrimp, and then placed in seawater. Survival rate of shrimp immersed in seawater containing the powder, filtrate, residue, and extract was significantly higher than in control shrimp after 24-120 h. Phagocytic activity and clearance efficiency against V. alginolyticus were significantly higher in shrimp receiving the powder, filtrate, residue, and extract than in control shrimp. It was concluded that shrimp hemcytes incubated with U. lactuca powder, its filtrate, residue, and extract activated the prophenoloxidase system, and shrimp immersed in seawater containing the powder, filtrate, and residue at 500 mg/l, and extract at 200 mg/l had increased immunity and resistance against V. alginolyticus infection.
並列摘要
白蝦（Litopenaeus vannamei）給與石蓴（Ulva lactuca）粉末、及其濾液、殘留物、萃取物，以體外和體內實驗，研究原酚氧化酵素活性、免疫力、溶藻弧菌抵抗。在體外實驗，白蝦血淋巴球經石蓴粉末、及其濾液、殘留物、萃取物培養，顯示酚氧化酵素（phenoloxidase）活性及超氧離子（superoxide anion）增加。白蝦浸泡500 mg/l石蓴粉末、及其濾液、殘留物，200 mg/l萃取物，經1，3，5小時，檢測透明球（hyaline cell），顆粒球（granular cell，包含半顆粒球），總血球（total hemocyte count），酚氧化酵素活性及超氧離子，發現經3－5小時後，免疫因子均顯著高於控制組。白蝦浸泡石蓴粉末、及其濾液、殘留物、萃取物，經3小時後，感染溶藻弧菌1 × 10^6 cfu（colony-forming unit），放置於海水，觀察存活率。發現經接受石蓴物質後的白蝦，經24－120小時，其存活率均大於控制組。白蝦接受石蓴粉末、及其濾液、殘留物、萃取物，其吞噬作用（phagocytic activity）和清除能力（clearance efficiency）顯著高於控制組。實驗証實白蝦血球接受石蓴粉末，及其濾液，殘留物，萃取物，活化原酚氧化酵素系統。白蝦浸泡於含石蓴粉末500 mg/l、及其濾液、殘留物、以及浸泡200 mg/l萃取物，提升免疫力，抵抗溶藻弧菌感染。