碳氮源對兩種白腐菌處理紙漿漂白廢水效能之影響

Impact of Carbon and Nitrogen Sources on Pulp Bleaching Effluent Treatment Efficiencies by Two White-Rot Fungi

蕭英倫(Ing-Luen Shiau) ； 陳清環(Ching-Huan Chen) ； 王亞男(Ya-Nan Wang)

《中華林學季刊》 41卷3期 (2008/09) Pp. 413-423

https://doi.org/10.30064/QJCF.200809.0009

摘要

製漿造紙廢水中偏高的色度，會影響承受水體的外觀。而製漿漂白過程中產生的木質素及其衍生物則是此等色度之來源，然而一般微生物及傳統廢水處理流程卻難以處理此等含木質素及酚基之廢水，因此能分解之木質素系污染物的白腐菌應用日益受到重視。然而白腐菌所分泌之木質素分解酵素系統，其誘發受到培養環境基質中碳、氮養分含量的影響。有鑑於此，本研究藉由兩種白腐菌Phanerochaete chrysosporium與Trametes versicolor非專一性之分解能力，來處理硫酸鹽紙漿漂白廢水，並針對碳氮源的變化影響其處理效能加以探討，探討五種不同碳源：glucose、fructose、starch、sucrose和未漂闊葉樹硫酸鹽紙漿作為共代謝基質之對脫色效果之影響，並選出最佳的碳源變化其濃度，以及0~1.5g/L之NH4NO3氮源，探討其對脫色效果之影響。由實驗結果顯示，適合Phanerochaete chrysosporium ATCC 36319處理的共代謝碳源基質為glucose和starch、碳源濃度為5 g/L、NH4NO3濃度為0.1 g/L；而Trametes versicolor ATCC 37501處理的共代謝碳源基質為glucose和starch、碳濃度10 g/L、NH4NO3濃度為0.2 g/L。

關鍵字

脫色 ； Phanerochaete chrysosporium ； 紙漿漂白廢水 ； Trametes versicolor ； 白腐菌

並列摘要

The pulp and paper mill effluent is highly colored, imparting black/brown color to the water body. The color is mainly due to lignin and its derivatives released during various stages in the pulping and bleaching process. The complex nature of such lignin compounds and their phenolic nature make them extremely resistant to microbial degradation and conventional treatment methods. Hence, the applications of lignin-degrading fungi are getting more attention. However, the inductions of lignin-degrading systems possessed by white fungi are dictated by carbon and nitrogen contents in the media. This study employed unspecific degrading capabilities of Phanerochaete chrysosporium and Trametes versicolor to treat kraft pulp bleaching effluents. Impacts of carbon and nitrogen genres and concentration on effluent decolourization were investigated with five carbon sources (as co-substrates): glucose, fructose, starch, sucrose and unbleached hardwood kraft pulp, as well as 0~1.5 g/L NH4NO3 as nitrogen source. The optimal carbon source and its concentration were identified. Results demonstrated that the optimal co-substrates carbon and nitrogen sources for Phanerochaete chrysosporium were glucose and starch at 5 g/L, NH4NO3 at 0.1 g/L; while for Trametes versicolor glucose and starch at 5 g/L, NH4NO3 at 0.2 g/L, respectively.

並列關鍵字

Decolorization ； Phanerochaete chrysosporium ； Pulp bleaching effluent ； Trametes versicolor ； White-rot fungi