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開發尼羅草專一性分子標記

Development of Specific Molecular Markers for Nilegrass (Acroceras macrum Stapf)

摘要


Nilegrass (Acroceras macrum Stapf) is one of major perennial forage grasses in Taiwan. It possesses many elite agronomic traits, i.e., high forage yield, high crude protein, rust resistance and low brown leaf percentage. The general variety identification indexes are a series of morphological traits such as plant height and leaf number. Vegetative propagation crop was frequently caused by gene loss by cuttage technique. It was the most important goal to protect the new variety by development of variety identification methods. The morphological discriminable power is lower than molecular marker because the agronomic traits are effected by environment factors and the interaction between genes and the environment. In order to protect plant breeder rights, we needed to develop a molecular identification technique for nilegrass hybrid lines. In this study, six elite introduction lines and 11 hybrids were tested. 81 ISSR-CAPS markers were used to get a ISSR PCR product combined with a restriction enzyme for molecular identification tool in a general strategy. The results showed that the average genetic similarity (GS) was 0.42 for all test nilegrass lines. AC30 and AC29 possessed the lowest GS with a group of hybrids, i.e., A02, A05, A06 (GS<0.15). It showed higher GS within the group of hybrids, i.e., A02, A05, A06 (GS>0.9). In parentage analysis of all hybrid lines, the results showed that AC26 was the most possible male parent for A02 (4.54), A05 (4.54), A06 (4.54) and A07 (4.66); AC29 for A01 (4.71) and A04 (4.74); AC30 for A03 (4.62), A08 (4.83), A09 (4.55) and A11 (4.59), respectively. Further, the results showed that the minimum sets of 3 ISSR-CAPS treatment might identify all elite nilegrass lines except the combination between NLT1 and AC32.

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