- 期刊
Temporal and Spatial Expression of Hypoxia-Inducible Factor-1alpha and Vascular Endothelial Growth Factor in a Rat Model of Myocardial Ischemia with or without Reperfusion
並列摘要
Background and Purpose: Although hypoxia-inducible factor-1alpha (HIF-1α) plays a major role in the prevention of myocardial ischemia, the temporal and spatial patterns of expression of HIF-1α in myocardial ischemia-reperfusion are not well known. This study examined the role of HIF-1α and vascular endothelial growth factor (VEGF) in myocardial ischemia-reperfusion. Methods: Adult Wistar rats were studied after ligation of the left anterior descending coronary artery (LAD) for 30 min and them after reperfusion. HIF-1α and VEGF were measured immediately after relief of occlusion and at 30 min, 1, 3, 6, and 24 h after reperfusion, HIF-1α and VEGF proteins were also measured 6 h after permanent occlusion of the LAD. Results: HIF-1α and VEGF mRNA increased 1.8- and 1.4-fold, respectively, immediately after relief of occlusion and reached a maximum of 4.3- and 2.3-fold, respectively, at 3 h after reperfusion and remained elevated up to 24 h. HIF-1α and VEGF proteins increased immediately after relief of ischemia. HIF-1α protein significantly increased from 0.5 h to 24 h after reperfusion and VEGF protein significantly increased form 1 h to 6 h after reperfusion compared to the sham control. Administration of HIF-1α antisense oligonucleotide before ligation of the LAD significantly inhibited VEGF protein expression induced by ischemia-reperfusion. Immunohistochemical study showed increased immunoreactivity of HIF-1α and VEGF in the jeopardized myocardium after ischemia-reperfusion HIF-1α and VEGF proteins were increased at 6 h after permanent occlusion of the LAD. Conclusions: This study demonstrated that HIF-1α and VEGF were co-induced in a temporal and spatial pattern after ischemia-reperfusion in the rat ventricular myocardium.
延伸閱讀
- 胡佩欣（2011）。Hypoxia enhances the activities of vascular endothelial growth factor and matrix metalloprotease-9 in cultured human retinal pigment epithelium cell in vitro〔碩士論文，高雄醫學大學〕。華藝線上圖書館。https://doi.org/10.6832/KMU.2011.00112
- 陳淑貞（2004）。Mechanisms of The Induction of Vascular Endothelial Growth Factor Expression by Hyperbaric Oxygen in Human Umbilical Vein Endothelial Cells〔碩士論文，臺北醫學大學〕。華藝線上圖書館。https://www.airitilibrary.com/Article/Detail?DocID=U0007-1704200714553709
- Shi, Y. (2006). Endothelial dysfunction and changes in vascular smooth muscle responsiveness in femoral arteries of rats with type I diabetes [doctoral dissertation, The University of Hong Kong]. Airiti Library. https://www.airitilibrary.com/Article/Detail?DocID=U0029-1812201200014220
- Lin, C. F. (2020). Investigation of the pathogenic factors for cardiac fibrosis after myocardial infarction and potential effects of histone deacetylase inhibitors on cardiac ischemia-reperfusion injury [doctoral dissertation, Taipei Medical University]. Airiti Library. https://www.airitilibrary.com/Article/Detail?DocID=U0007-2712201914422200
- lin, C. H. (2010). The signaling mechanism of lysophospholipids-regulated vascular endothelial growth factor-C and lymphatic marker expressions in human umbilical cord vein endothelial cells [master's thesis, National Taiwan University]. Airiti Library. https://doi.org/10.6342/NTU.2010.00607