雲南石梓之試管內營養系繁殖與細胞懸浮培養

In Vitro Clonal Propagation and Cell Suspension Culture of Gmelina aborea R.

楊政川(Jenq-Chuan Yang) ； 蔡錦瑩(Jeen-Yin Tsay) ； 鍾振德(Jeng-Der Chung) ； 陳振榮(Zenn-Zong Chen)

《林業試驗所研究報告季刊》 8卷1期 (1993/03) Pp. 1-9

https://doi.org/10.7075/BTFRI.199303.0001

摘要

雲南石梓種子經破除堅硬的內果皮後，已使發芽的困難度降低。取無菌苗之節、節間、葉片、及子葉分別培養於添加不同NAA與BA濃度之MS培養基誘導多芽體。其中以節培養在MS培養基添加0.01mg/l NAA與5mg/l BA的處理效果最佳。平均每個培植體可生產3.5個芽體。培養基添加0.2%的活性碳可促進芽體的抽長。截取0.5至1.5cm之芽體，置於添加4種IBA濃度之MS培養基中誘導發根，71%的芽體在添加4mg/l的培養基中被誘導發根，但是此種濃度的IBA會影響根的發育形態。當培養基僅添加1mg/l IBA時，芽體的發根率雖然只有50%，惟根系良好，且根的形態與種子苗者相似。截取的芽體也被直接扦揷於（石至）石、泥炭土及珍珠石(2:2:1)組成的介質發根。雖然此種管外發根的方法僅可獲取63.9%的發根率，而且短於1cm的芽體難以發根，但是在大量生產小植株提供營養系造林上頗具應用價值。此外，截取自子葉、下胚軸、葉片及節的培植體置於分別添加IBA、NAA或2，4-D的MS培養基皆可誘導癒合組織的形成。衍自癒合組織的細胞懸浮培養亦已建立，且最佳的細胞增殖處理為液體MS培養基添加3mg/l NAA與1mg/l BA。

關鍵字

多芽體再生 ； 小植株生產 ； 懸浮培養 ； 雲南石梓

並列摘要

The germination difficulty in Gmelina aborea R. was improved by removal of the stony endocarps. Nodes, internodes, leave pieces, and cotyledons were cultured on MS media containing various concentrations of NAA and BA. The best treatment for multiple shoot production was with nodes plated on a MS medium containing 0.01mg/l NAA and 5mg/l BA in which 3.5 shoots per node were regenerated on the average. Shoot elongation was promoted by adding 0.2% activated charcoal. To produce plantlets, shoots were excised to 0.5 to 1.5cm long and rooting was induced on MS media containing 4 different concentrations of IBA. Seventy one percent of the shoots were successfully rooted on an MS medium containing 4mg/l IBA. The high IBA concentration influenced root development. At a level of 1mg/l IBA, rooting percentage was only 50%, but a fine root system was obtained. The root morphology of the fine root system was apparently similar to that of seedlings. Excised shoots were also rooted on a growth medium consisting of vermiculite, peat and perlite (2:2:1). Although only 63.9% of shoots were induced to root and shoots shorter than 1cm were hardly rooted ex vitro, the method would be quite useful for mass plantlet production as is required for practical clonal reforestation. Callus induced from cotyledon, hypocotyl, leaf and node was successfully plated on MS media containing only IBA, NAA, or 2,4-D. Suspension cultures derived from this callus were successfully established. Cell proliferation was optimal in MS liquid medium containing 3mg/l NAA and 1mg/l BA.

並列關鍵字

Multiple shoot regeneration ； Plantlet production ； Suspension culture ； Gmelina aborea