Occurrence and Recovery of Vitrification in Tissue Cultures of Paulownia Species

泡桐類組織培養玻璃質化之發生與恢復

何政坤(Chcng-Kucn Ho) ； G. Jacobs

《林業試驗所研究報告季刊》 10卷4期 (1995/12) Pp. 391-403

https://doi.org/10.7075/BTFRI.199512.0391

摘要

泡桐(P. fortunei)、白桐(P. kawakamii)、台灣泡桐(P. x Taiwaniana)與毛泡桐(P. tomentosa)之節間培植體分化之不定芽的玻璃質化(vitrifyication)研究顯示：不同深度的洋菜粉(agar)、鈷(cobalt)、肌醇(inositol)、MS培養基巨量元素及生長激索與不同光照强度不能有效消除不定芽所産生之玻璃質化，雖然增加光照强度可提高芽體的增殖率。台灣泡桐芽體的玻璃質化比泡桐與白桐較不易形成水晶凝膠(gelrite)比洋菜粉凝膠對芽體之增殖有促進作用但較易形成玻璃質化芽體。正常與玻璃質芽體縱代在含不同凝膠質體與濃度之培養基，由二者之形態與生長量發現：泡桐與毛泡桐玻璃質芽在芽體基部切口形成較多的癒傷組織，而台灣泡桐與白桐則易抽出側芽。玻璃質芽較正常芽有較高的生長量且具有相同之發根力。增加凝膠質體濃度會抑制泡桐芽體的生長，但白桐先天生長較慢，在各種濃度之生長表現均無差異。玻璃質芽培養在0.8%或以上濃度之Difco洋菜粉凝膠培養基，可降低芽體含水量且恢復成正常芽之生長。

關鍵字

凝膠質 ； 泡桐類 ； 組織培養 ； 玻璃質化

並列摘要

Vitrification of adventitious buds induced from internodal explants of Paulownia fortunei, P. kawakamii. P. tomentosa and P. x taiwaniana was studied. Different concentrations of agar, cobalt, inositol, MS macrosalts, growth regulators and different light intensities did not effectively eliminate vitrification, however, increasing light intensity actually increased adventitious bud formation, P. x taiwaniana formed fewer vitrified shoots than P. fortunei and P. kawakamii. Gelrite induced more adventitious buds but caused more vitrification than did agar. The morphological characteristics and growth of normal and vitrified shoots were studied when subcultured on different gelling agents and at different concentrations. Vitrified shoots of P. tomentosa produced more calli on the cut surfaces of shoots whereas the other two species formed more axillary shoots. Vitrified shoots had a higher growth rate than normal shoots but the capability to form roots did not diffcr from normal shoots. Incrcasing the concentration of gelling agcnts inhibited shoot growth except with P. kawakamii which had an inherently slower growth rate on all concentrations of gelling agents tested. Vitrified shoots decreased in water content and reverted to normal shoots when cultured on Difco agar at 0.8% or higher, but this was not seen with gelrite nor mixtures of gelrite and agar.

並列關鍵字

gelling agents ； Paulownia species ； tissue culture ； vitrification