- 期刊
- OpenAccess
Both U-74006F and Dexamethasone Inhibit Interleukin-1β Induced Vascular Smooth Muscle Oscillations
並列摘要
Background: Incubation of aortic rings with interleukin-1β (IL-1β) induces spontaneous oscillation during phenylephrine-induced contraction. We hypothesized that the steroid compounds, U-74006F and dexamethasone could inhibit the induction of inducible nitric oxide synthase (iNOS) and further inhibit this oscillation. Methods: Rat aortic rings (endothelium removed) were incubated in IL-1β with or without the non-glucocorticoid 21-aminosteroid U-74006F (10^(-6) M) or the glucocorticoid dexamethasone (10^(-6) M) for 3, 5 or 7 hours. Spontaneous oscillation was induced by contraction with phenylephrine. Results: Oscillation occurred during phenylephrine-induced contraction and Larginine-induced relaxation (indicator of iNOS activity) in IL-1β incubated vessels. Pretreating the vessels with N^ω-nitro-L-arginine (10^(-4) M) did not significantly inhibit the phenylephrine-induced oscillation in IL-1β incubated vessels suggesting a non-iNOS mechanism involved in inducing oscillation. The oscillation frequency and magnitude were significantly inhibited by both dexamethasone and U-74006F. Dexamethasone incubation inhibited L-arginine-induced relaxation and augmented phenylephrine-induced contraction when compared to IL-1β incubated vessels which was not observed in U-74006F incubated vessels. Conclusions: U-74006F may have a therapeutic advantage over dexamethasone in that U-74006F could inhibit pathological oscillation activity with minor effects on L-arginine induced relaxation, and contractile responses to phenylephrine.
延伸閱讀
- Morand, E. F., Rickard, D., & Goulding, N. J. (1992). Lack of involvement of lipocortin 1 in dexamethasone suppression of IL-1 release. Mediators of Inflammation, 1993(), 49-52. https://doi.org/10.1155/S0962935193000067
- Lin, F. Y. (2006). Botulinum Neruotoxin Type A Inhibits Neurogenic Inflammation at Pre-Vascular Level [master's thesis, Taipei Medical University]. Airiti Library. https://www.airitilibrary.com/Article/Detail?DocID=U0007-1704200715021113
- 柯瀞雅（2010）。Mechanism of the Inhibitory Effect of Hinokitiol on Rat Vascular Smooth Muscle Cell Proliferation〔碩士論文，臺北醫學大學〕。華藝線上圖書館。https://doi.org/10.6831/TMU.2010.00090
- 張雯鈞（2012）。The inhibition of 1-Deoxynojirimycin hydrochloride (DNJ) on high glucose induces migration in vascular smooth muscle cell〔碩士論文，中山醫學大學〕。華藝線上圖書館。https://www.airitilibrary.com/Article/Detail?DocID=U0003-2708201211075600
- 李宜馨（2011）。Mechanism of the inhibitory effect of hinokitiol on vascular smooth muscle cell proliferation〔碩士論文，臺北醫學大學〕。華藝線上圖書館。https://www.airitilibrary.com/Article/Detail?DocID=U0007-2107201116193500