- 期刊
Up-Regulation of Phosphorylated ATM/ATR Substrate/Akt Expression by Phenylephrine in Peri-Infarct Myocardium in Rats
並列摘要
Purpose: To investigate the changes of phosphorylated ataxia-telangiectasia-mutated (ATM)/ATM and Rad3-related (ATR) substrate and Akt proteins (p-ATM/ATR substrate and p-Akt) and their regulations by phenylephrine (PE) in the peri-infarct myocardium in rats.Methods: Myocardial infarction (MI) and Sham-operation were established by Litwin's method. Three days after operation, surviving Sprague-Dawley male rats were divided into four groups: MI, Sham, MI+PE, and Sham+PE group. Physiological buffered saline (1 ml/kg) or PE (0.65 mg/kg) were injected into the peritoneal cavity every day for 8 weeks, respectively. Twelve weeks after treatment, p-ATM/ATR substrate and p-Akt expression in myocardium around the infarcted regions were detected by Western blot.Results: Twelve weeks after treatment, p-ATM/ATR substrate and p-Akt expression were detected through Western blot in myocardium around the infarcted regions. The intensity (represented as normalized integrated optical density, IOD) for p-ATM/ATR substrate and p-Akt were (0.59±0.07 and 0.68±0.03) in the MI group, (0.63±0.05 and 0.72±0.04) in the Sham group, (0.99±0.07 and 1.03±0.05) in the MI+PE group and (0.65±0.04 and 0.75±0.04) in the Sham+PE group, respectively. Both presented with significantly increased expression in the MI+PE group as compared with the Sharn+PE group (p＜0.05); and in the MI+PE group as compared with the MI group (p＜0.05). No difference was observed between the MI and Sham groups, or between the Sham+PE and Sham groups (p＞0.05).Conclusion: PE can significantly up-regulate p-ATM/ATR substrate and p-Akt expression and thus might activate ATM/ATR substrate/Akt pathway in myocardium around the infarcted regions. The activated ATM/ATR substrate/Akt pathway might be associated with the attenuation of post-infarction cardiac remodeling, fibrosis, ischemic cardiomyopathy and heart failure induced by PE.
延伸閱讀
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