Effects of Dexamethasone and Lovastatin on the Expression of BMP-2 in Bone Marrow Stroma Cells Cultured from Patients with Osteonecrosis

Glucocorticoids have been reported to shift the properties of osteogenesis to adipogenesis in murine pluripotent marrow stroma cells. These effects were thought to be one of the important mechanisms to induce osteonecrosis. Statins, HMG-CoA reductase inhibitors, were found to be able to reverse the effect of steroids on osteogenesis of stroma cells and to prevent the osteonecrosis in chicken. However those effects have not been confirmed in human marrow stroma cells. This study was designed to confirm these effects of steroids and statins on osteogenesis in human stroma cells and compare the differences of the effects between osteonecrotic patients and non-osteonecrotic person. Human bone marrow stroma cells were obtained and cultured from iliac crest of 3 patients with osteonecrosis of the femoral heads and 4 age controlled patients with dysplastic osteoarthritis or trauma of the hip. After 4 days treatments with dexamethasone (10^(-7)M), lovastatin (10^(-6)M) or combined treatment of Dexamethasone (10^(-7)M) and Lovastatin (10^(-6)M), bone morphogenetic protein-2 (BMP-2) mRNA expression were examined by RT-PCR. Our results showed that dexamethasone suppressed BMP-2 expression in 3 osteonecrosis cases (P＜0.05) and only 20% suppressed in 5 non-osteonecrosis cases (non-significant). Lovastatin increased BMP-2 mRNA expression in both groups (P＜0.01). The reverse effect of lovastatin on dexamethasone was noted in both groups, however the osteonecrosis group was less effective.

並列關鍵字

steroid ； statin BMP2 ； osteogenesis ； and osteonecrosis

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