老化促進小鼠於不同月齡之老化變化研究

Studies of Aging in Senescence-accelerated Mice at Different Ages

吳青蓮(Ching-Lein Wu) ； 詹吟菁(Yin-Ching Chan) ； 陳英茹(In-Lu Chen) ； 王銘富(Ming-Fu Wang)

《中華民國營養學會雜誌》 26卷4期 (2001/12) Pp. 248-257

https://doi.org/10.6691/JCNS.200112_26(4).0004

摘要

The purpose of this study was to examine variations in learning and memory and brain spongy degeneration with age in senescence-accelerated mice (SAM). Senescence-accelerated mice (P8 strain) were divided into five age groups (containing mice at 3, 6, 9, 12, and 15 months old), and each group included 20 male and 20 female mice. Animals were fed a general solid diet; body weight and food intake were recorded and open field activity test and single-trial passive avoidance were evaluated during the experiment. After the experiment, mice were sacrificed to evaluate the brain histopathology of spongy degeneration. The results show that the 9-month-old group had the highest food intake (p ＜ 0.01). Body weights of the 12- and 15-month-old mice in the single-trial passive avoidance test (p ＜ 0.05) for males. However, in female groups, 15-month-old mice had significantly lower learning ability and memory (p ＜ 0.05). Spongy degeneration of the brain became more serious with age. Vacuoles were mainly observed in the brain stem and spinal cord section, and increased from front to back. To sum up, the results indicate that learning and memory abilities decreased and brain spongy degeneration became more serious with age. Therefore, we conclude that the results of this study may serve a model for future aging studies.

關鍵字

SAMP8系小鼠 ； 學習記憶 ； 海綿樣變性

並列摘要

The purpose of this study was to examine variations in learning and memory and brain spongy degeneration with age in senescence-accelerated mice (SAM). Senescence-accelerated mice (P8 strain) were divided into five age groups (containing mice at 3, 6, 9, 12, and 15 months old), and each group included 20 male and 20 female mice. Animals were fed a general solid diet; body weight and food intake were recorded and open field activity test and single-trial passive avoidance were evaluated during the experiment. After the experiment, mice were sacrificed to evaluate the brain histopathology of spongy degeneration. The results show that the 9-month-old group had the highest food intake (p ＜ 0.01). Body weights of the 12- and 15-month-old mice in the single-trial passive avoidance test (p ＜ 0.05) for males. However, in female groups, 15-month-old mice had significantly lower learning ability and memory (p ＜ 0.05). Spongy degeneration of the brain became more serious with age. Vacuoles were mainly observed in the brain stem and spinal cord section, and increased from front to back. To sum up, the results indicate that learning and memory abilities decreased and brain spongy degeneration became more serious with age. Therefore, we conclude that the results of this study may serve a model for future aging studies.

並列關鍵字

senescence-accelerated mice ； learning and memory ； spongy degeneration