具抗病性野生種木瓜組織培養保存之研究

Studies on the Preservation Using Tissue Culture of PRSV-resistant Wild Species of Papaya

黃柄龍(Ping-Lung Huang) ； 廖麗貞(Li-Jen Liao) ； 萬雄(Hsiung Wan)

《高雄區農業改良場研究彙報》 13卷2期 (2002/06) Pp. 13-25

https://doi.org/10.6958/RBKDAIS.200206.0013

摘要

野生種木瓜Carica goudotiana的葉柄培植體在含1.0mg/l NAA、0.5mg/l kinetin及1.0mg/l GA3的1/2 MS培養基上，可經由誘導的癒合組織直接產生體胚，其體胚形成能力約為50%；且所長出的根，亦可於此D1培養基中誘得癒合組織及25%的體胚形成，此癒合組織並可於WL（White's）之固態分化培養基中誘得體胚分化。所得似胚軸組織於添加0.5mg/l IBA之MS培養基中，可促進癒合組織及體胚形成。Carica pubescens經以D1誘導培養基所得之葉柄癒合組織，其體胚分化能力僅發生於EL4及WL之固態分化培養基中（5～6.7%）。所形成的未萌芽體胚，可在含0.001ppm BA之EM（embryo mature）培養基中成熟、萌發。萌芽的體胚不需經由任何的誘根處理，即能長成具有健全根系的植株。

關鍵字

木瓜 ； 野生種 ； 組織培養

並列摘要

The petiole explant of wild species Carica goudotiana cultured in 1/2MS medium containing 1.0mg/l NAA, 0.5mg/l kinetin and 1.0mg/l GA3 could produce somatic embryo directly through the induced callus. The frequency of somatic embryo formation was about 50%. The root tissue from somatic embryo could also produce callus and induce embryogenesis with a frequency of 25% in D1 medium. These calli could further differentiate into somatic embryo on solid differentiation medium of WL (White's). Upon transferring the hypocotyl-like tissue obtained from D1 medium to MS medium containing 0.5mg/l IBA, induction of callus and somatic embryo was also recorded.The callus produced from petiole explant of Carica pubescens cultured in D1 medium was low in embryogenesis capability. However, embryogenesis percentage was increased by 5~6.7% when callus was cultured on EL4 or WL solid differentiation media. The somatic embryos obtained from EL4 and WL media could mature and germinate after transferring to EM (embryo mature) medium containing 0.001mg/l BA. The germinated somatic embryos could develop into intact plants with vigorous root system.

並列關鍵字

C. papaya ； Wild species ； Tissue culture