- 期刊
Modification of Protein Release From Microparticles Coated by Chitosan
並列摘要
This study was designed to overcome the initial burst release of bovine serum albumin (BSA) from poly(ε-caprolactone) (PCL) microparticles via coating with a hydrophilic polymer, chitosan. The microparticles were prepared by hot-melt technique following coated with different concentrations of chitosan solutions. The influences of coating material on the surface morphology as well ass particle size of microparticles, protein entrapment, and in vitro release property were investigated. The mean particle sizes of chitosan-coated microparticles were in the range of 16.6±0.5 ~ 18.6±0.4 μm, and all of these values were larger than those of uncoated microparticles. The entrapments of protein were 4.63 ± 0.03% - 4.73 ± 0.03% in uncoated micropartic1es and 2.64±0.08% ~ 3.91 ± 0.11 % in coated microparticles. In vitro release study showed that chitosan significantly reduced the initial burst release and the cumulative amount of BSA released at the end of 7 days (p ＜ 0.05). It was because that when water was attracted inside the matrix leading chitosan swollen and formed a barrier to delay protein release. Furthermore, the diffusion of protein direct from intertanglement structure of swollen polymer was much difficult due to its size.