Influence of Flavonoids on Regulation of MMPs Activity in THP-1 Cells

Many natural products as well as synthetic compounds have been shown to be potent cancer prevention agents against chemically-induced tumors in animals. One of the early events involves proteolytic degradation of extracellular matrix components, and the concept that particular pro- teases, especially matrix metalloproteinases (MMP), contribute to invasion and metastasis of cancer cells has been well accepted. Suppression of MMPs gene induction is related to inhibition of cancer invasion. Therefore, our study focused on the regulation of MMPs by flavonoids in THP-1 cells by protein kinase C (PKC) activation. All the flavonoids used showed slightly toxicity at the concentration (50μg/mL) used for MMP activity assay. Phorbol-12-myristate-13-acetate (PMA) increased MMP-1, MMP-2, and MMP-9 activities. Pretreatment of cells with quercetin, apigenin, daidzin, and lutolin inhibited these three MMPs activities induced by PMA. Hesperitin reduced MMP-1 activity induced by PMA. Other flavonoids had no effect on MMP activity. Quercetin, taxifolin, apigenin, daidzin, lutolin, and hesperitin inhibited THP-1 cells migration was also observed as a functional assay. In conclusion, several flavonoids possess the ability to reduce MMP activity which then in turn inhibiting THP-l migration that might related with cancer progression.

並列關鍵字

THP-1 ； Flavonoid ； MMP ； Chemoprevention

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