Paternity Testing with DNA Fingerprinting: Report of One Case

DNA 指紋作為親子鑑定之應用：一病例報告

蔡立平(Li-Ping Tsai) ； 胡務亮(Wuh-Liang Hwu) ； 方麗容(Li-Jung Fang) ； 王作仁(Tso-Ren Wang) ； 江千代(Chien-Dai Chiang)

《慈濟醫學雜誌》 8卷1期 (1996/03) Pp. 61-65

https://doi.org/10.6440/TZUCMJ.199603.0061

摘要

實驗室檢查一例血小板低下症新生兒，發現無法解釋的結果，促使我們應用DNA指紋做親子鑑定。同位素32P-dATP標定的寡核苷酸（GTG）5作探針，與限制酶Hinf I 分解後的雙親和病童DNA雜交。結果顯示沒有任何一條雜交帶是由父親傳給病童，同時病童有三條雜交帶，在雙親的相對位置上均沒發現。所以DNA指紋認定非親生父親。對本個案進行親子鑑定，不僅可以解決我們臨床上疑慮，同時也提供我們對下次懷孕是否須進行特殊週產期照顧的參考，每一個人都有自己特定的DNA指紋，臨床上應用於個體的鑑定，可以提供十分完的訊息，遠較傳統性的方式為佳，親子鑑定自然也不例外。

關鍵字

DNA 指紋 ； 親子鑑定

並列摘要

The paternity of a newborn with immune thrombocytopenia with unexplained laboratory data was questioned. DNA fingerprinting was employed to test the paternity of the patient. Oligonucleotide (GTG)5 probe labeled with radioactive r-32P-ATP was used to analyze Hinf I digested high molecular weight DNA from the patient and the parents. The results showed that, in the gel, no polymorphic band was specifically transmitted from the putative father to patient, and three bands of the patient could not be traced back to either of the parents. So, non-paternity was confirmed. Such identification not only supported the clinical suspicion, but also directed greater perinatal care in future pregnancies. DNA fingerprinting, unique to each individual, is a powerful technique and con be applied directly to problems of individual identification, including parentage testing.

並列關鍵字

DNA fingerprinting ； parentage testing