A 57-year-old female presented with intermittent painless gross hematuria for several weeks. In the preoperative evaluations, including intravenous urography, retrograde pyelography, and computed tomography, transitional cell carcinoma of the renal pelvis of the left atrophy kidney was highly suspected. After a transperitoneal laparoscopic left nephroureterectomy, the bladder cuff was excised and intracorporeally repaired, exclusively using the free-hand suture-and-knotting technique. The specimen was retrieved via an Endo Pocket(superscript ®) (Unimax Medical Systems, Taiwan) through a 3.5-cm Pfannestiel incision. Her postoperative recovery was prompt and excellent. Therefore, a total laparoscopic nephroureterectomy with bladder cuff excision, in selected cases completely carried out intracorporeally while adhering to all oncological principles, is efficacious and safe, with minimal invasiveness.