Stripe rust is one of the most destructive diseases of wheat crops in China. Two rust physiological strains, i.e., CYR30 (Intern. name: 175 E 191) and CYR31 (Intern. name: 239 E 175) have been the dominant and epidemic physiological strains since 1994. In order to exploit elite resistant germplasm for wheat breeding in China, 26 synthetic hexaploid wheat (Triticum turgidum ×Aegilops tauschii) were evaluated at the adult stage for resistance to the Chinese stripe rust physiological strain CYR30 and CYR31. Of 26 synthetic hexaploids, 19 lines displayed resistance to the target diseases, which provide new and promising resistant resources for wheat breeding in China. In order to analyze genetic expression and suppression of the resistant genes derived from the parents in the synthetics, the stripe rust infection types (ITs) of 13 synthetics and their parental species, i.e., five T. turgidum wheat varieties and 12 accessions of Ae. tauschii, were compared. Most of the resistant genes derived from T. turgidum or and Ae. tauschii were expressed in the synthetic hexaploids. However, the suppression occasionally occurred in both of the A or B and D genomes of T. turgidum and Ae. tauschii, respectively. The suppression of resistant genes seemed to be donor specific either to T. turgidum or Ae. tauschii in synthetic hexaploids. The methodology to overcome the resistance suppression was discussed.