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Circadian Expressions of Starch Branching Enzyme Gene in Sweet Potato Leaves

並列摘要


Diurnal changes of transcript levels of starch branching enzyme (SBE) involved in building tip of amylopectin structure were detected in sweet potato leaves during 16 h light/8 h dark (16 L/8 D,) photoperiod cycles, and the accumulation peak of SBE mRNA presented at the end of light period. Furthermore, the fluctuation of the transcript levels in leaves was still remained when the 16 L/8 D adapted plants were shifted to the 24-h constant light condition, just the periodic length of gene expression in constant light was slightly shorter than that in 16 L/8 D. These results indicated that the diurnal oscillation of SBE transcripts could be controlled by an endogenous biological clock. However, the expression of SBE gene presented the different diurnal rhythm with that of granule-bound starch synthase I gene that was in charge of amylose synthesis. To understand the circadian regulations of SBE gene, an effort was made to isolate and characterize the SBE promoter. Several putative circadian and light regulatory elements were found in the SBE promoter sequence.

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