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  • 期刊

過敏性氣喘寒熱證型動物模型之建立

Allergic Asthma Animal Model with Cold and Hot "Zheng"

摘要


”Zheng” is the unique theory of diagnosis and treatment in Traditional Chinese Medicine (TCM). According to this theory, the acute phase of asthma is divided into cold ”Zheng” and hot ”Zheng”. The aim of this study was to establish an asthma murine model with cold ”Zheng” and hot ”Zheng” to evaluate the efficacy of TCM for the treatment of bronchial asthma and to study the mechanisms. First, we fed Scutellariae Coptis Gardeniae and Aconitum Cinnamomum Zingiber separately to BALB/c mice to change their nature to cold and hot. In this experiment, the animals' appearances and changes of physical states, including body weight, body temperature, and heart rate were observed and recorded to estimate the effects of the Chinese herbal medicine. Then, we sensitized the mice using intraperitoneal injection of dust mite allergens (Dermatophagodes pteronyssinus group 5, Der p 5) and induced the allergen-specific airway hyperreactivity using inhalational challenges. Results of the pathological studies of the lung and trachea were observed to determine the severity of inflammation. The concentration of Der p 5-specific IgG and IgE antibodies in serum and cytokines IL-4 and INF-γ in bronchoalveolar larvage fluid were assessed using ELISA to clarify the differences of allergic immunoresponses between the animals with cold and hot ”Zheng”. We found that the animals with cold ”Zheng” and hot ”Zheng” were very different in appearance. The animals with hot ”Zheng” were much more active than the animals with cold ”Zheng”. According to the results of pathological examinations, the animals with hot ”Zheng” had the most severe airway inflammation as shown by the concentrations of both interleukin-4 and interferon-γ which were the highest among the three groups. Therefore, we suggest that the physiology and immunoresponses are quite different between the mice with cold ”Zheng” and hot ”Zheng”, and the production of IL-4 may play a role in the differences.

並列摘要


”Zheng” is the unique theory of diagnosis and treatment in Traditional Chinese Medicine (TCM). According to this theory, the acute phase of asthma is divided into cold ”Zheng” and hot ”Zheng”. The aim of this study was to establish an asthma murine model with cold ”Zheng” and hot ”Zheng” to evaluate the efficacy of TCM for the treatment of bronchial asthma and to study the mechanisms. First, we fed Scutellariae Coptis Gardeniae and Aconitum Cinnamomum Zingiber separately to BALB/c mice to change their nature to cold and hot. In this experiment, the animals' appearances and changes of physical states, including body weight, body temperature, and heart rate were observed and recorded to estimate the effects of the Chinese herbal medicine. Then, we sensitized the mice using intraperitoneal injection of dust mite allergens (Dermatophagodes pteronyssinus group 5, Der p 5) and induced the allergen-specific airway hyperreactivity using inhalational challenges. Results of the pathological studies of the lung and trachea were observed to determine the severity of inflammation. The concentration of Der p 5-specific IgG and IgE antibodies in serum and cytokines IL-4 and INF-γ in bronchoalveolar larvage fluid were assessed using ELISA to clarify the differences of allergic immunoresponses between the animals with cold and hot ”Zheng”. We found that the animals with cold ”Zheng” and hot ”Zheng” were very different in appearance. The animals with hot ”Zheng” were much more active than the animals with cold ”Zheng”. According to the results of pathological examinations, the animals with hot ”Zheng” had the most severe airway inflammation as shown by the concentrations of both interleukin-4 and interferon-γ which were the highest among the three groups. Therefore, we suggest that the physiology and immunoresponses are quite different between the mice with cold ”Zheng” and hot ”Zheng”, and the production of IL-4 may play a role in the differences.

延伸閱讀