中醫傳統方劑－柴胡疏肝散變方於四氯化碳誘導大鼠肝纖維化動物模式探討抑制肝纖維化之療效

A Study of the Antifibrotic Effects of Traditional Chinese Herbs Modified Chai-Hu-Shu-Gan Powder on Carbon Tetrachloride-Induced Liver Fibrosis in Rats

吳龍源(Long-Yuan Wu) ； 徐曉萍(Hsiao-Ping Hsu) ； 程景章(Ching-Chang Chen) ； 林乃女(Nai-Nu Lin) ； 蕭雅如(Ya-Ju Hsiao) ； 陳旺全(Wan-Chuan Chen) ； 鄭振鴻(Zheng-Huang Cheng) ； 徐慶霖(Chin-Lin Shyu) ； 王俊秀(Jiunn-Shiow Wang) ； 邱雲棕(Yung-Tsung Chiu)

《中醫藥雜誌》 15卷4期 (2004/12) Pp. 257-271

https://doi.org/10.6940/JCM.200412_15(4).03

摘要

The purpose of this study was to investigate the antifibrotic effects of modified Chai-Hu-Shu-Gan (CHSG) powder, comprised of Chinese herbs, on chemical-induced liver fibrosis in adult Sprague- Dawley rats. Liver fibrosis was induced by carbon tetrachloride (50 %, l.0 mL/kg, by gavage) twice a week for 8 weeks. Carbon tetrachloride-induced rats were randomly assigned to three groups: saline, low dose CHSG (1.26 g/kg) and high dose CHSG (6.30 g/kg), each given by gavage 5 times a week for 8 weeks, starting from the onset of carbon tetrachloride administration. Therapeutic effects were assessed by serum enzyme activities (AST and ALT), histopathological score of liver fibrosis, determination of liver collagen content and immunos-taining against smooth muscle cell α-actin to count the number of stellate cells. The content of α-smooth muscle actin was assayed by western blot, result shows that treatment with CHSG (6.30 g/kg) had a more significant reduction (p＜0.05) in the content of AST and ALT (132±38 U/L; 90±26 U/L) than carbon tetrachloride-induced rats receiving saline (240±130 U/L; 142±32 U/L). Liver collagen accumulation was also markedly reduced (p＜0.05) by the CHSG (6.30 g/kg) treatment. Histopathological examination revealed that the CHSG (6.30 g/kg) treatment significantly reduced the degree of liver fibrosis. In addition, it is found that the number of α-smooth muscle actin positive stellate cells and the content of α-smooth muscle actin were markedly reduced (p＜0.001) by CHSG (6.30 g/kg) treatment. These results show that modified Chai- Hu-Shu-Gan powder has anti-fibrotic effects on a carbon tetrachloride-induced liver fibrosi s rat model. Therefore, anti-fibrotic mechanisms might inhibit stellate cell proliferation and activation.

關鍵字

肝纖維化 ； 中醫傳統方劑 ； 肝臟星狀細胞 ； 動物模式

並列摘要

The purpose of this study was to investigate the antifibrotic effects of modified Chai-Hu-Shu-Gan (CHSG) powder, comprised of Chinese herbs, on chemical-induced liver fibrosis in adult Sprague- Dawley rats. Liver fibrosis was induced by carbon tetrachloride (50 %, l.0 mL/kg, by gavage) twice a week for 8 weeks. Carbon tetrachloride-induced rats were randomly assigned to three groups: saline, low dose CHSG (1.26 g/kg) and high dose CHSG (6.30 g/kg), each given by gavage 5 times a week for 8 weeks, starting from the onset of carbon tetrachloride administration. Therapeutic effects were assessed by serum enzyme activities (AST and ALT), histopathological score of liver fibrosis, determination of liver collagen content and immunos-taining against smooth muscle cell α-actin to count the number of stellate cells. The content of α-smooth muscle actin was assayed by western blot, result shows that treatment with CHSG (6.30 g/kg) had a more significant reduction (p＜0.05) in the content of AST and ALT (132±38 U/L; 90±26 U/L) than carbon tetrachloride-induced rats receiving saline (240±130 U/L; 142±32 U/L). Liver collagen accumulation was also markedly reduced (p＜0.05) by the CHSG (6.30 g/kg) treatment. Histopathological examination revealed that the CHSG (6.30 g/kg) treatment significantly reduced the degree of liver fibrosis. In addition, it is found that the number of α-smooth muscle actin positive stellate cells and the content of α-smooth muscle actin were markedly reduced (p＜0.001) by CHSG (6.30 g/kg) treatment. These results show that modified Chai- Hu-Shu-Gan powder has anti-fibrotic effects on a carbon tetrachloride-induced liver fibrosi s rat model. Therefore, anti-fibrotic mechanisms might inhibit stellate cell proliferation and activation.

並列關鍵字

Liver fibrosis ； Traditional Chinese herb ； Hepatic stellate cell ； Animal model