Compression garments have been widely used in the sport fields. In addition to the gradient compression, muscle support is the other major function for compression pants. However, current compression calf sleeves in the market seldom have both compression and muscle support function. This research designed the compression calf sleeves with progressive pressure and muscle support from biomechanics point of view, and investigated functional performance and perception tests of three different conditions (compression calf sleeves, CCS. normal calf sleeves, NCS. no wearing, NW). The purpose of this study was to compare the scientific data to analyze the effect of compression calf sleeves on performance. Nine healthy male participants (height 174.61±4.55 cm, weight 72.07±5.86 kg, age 24.33±1.58 years, calf girth 38.22±1.18 cm) were recruited to perform a single leg balance task with eyes-closed for up to 60 s to count the balance time and the area of center of pressure. Moreover, EMG signals were recorded for the tibialis anterior and gastrocnemius muscles during treadmill running at 10kph. Also, a 3-D accelerometer was taped on the gastrocnemius to measure the muscle vibration. In addition, the slope of the regression line of 30-time continuous vertical jump was determined to compare the magnitude of the vertical jump height decreased. Finally, the perception was recorded on comfort feelings and muscle support about CCS and NCS. The results showed that EMG significantly decreased at gastrocnemius in CCS compared to NW during running, and significant lower values of resultant and vertical acceleration were found in CCS. On the other hand, CCS had significantly lower slope of regression line than NW in 30-time continuous vertical jump tests. The perception tests of comfort feelings and muscle support also showed significantly improved in CCS. It was concluded that the CCS could accelerate blood circulation by progressive pressure and decrease muscle vibration by muscle support. The CCS could also increase the running economy and abbreviate fatigue. Besides, the CCS showed better subjective feelings than NCS.