分子拓印高分子感測器的製作與應用

Fabrication and Application of Molecularly Imprinted Polymer-Based Sensors

李世惠(Shih-Hui Lee) ； 董瑞安(Ruey-An Doong)

《科儀新知》 152期 (2006/06) Pp. 12-21

https://doi.org/10.29662/IT.200606.0002

摘要

現今生物感測器已被廣泛的應用於醫學診斷、環境監測和食品保鮮及污染檢測之目的，不同的生物分子如抗體、酵素及DNA均可被用來辨識目標物，但在現實的應用中，使用生物分子有許多的限制和困難，其中面臨的最大困難為部分目標物很難找到辨識的生物分子，且在高溫、高pH或有機溶劑等惡劣環境中，生物分子不容易穩定，導致生物感測器偵測效果不佳。而分子拓印高分子具有高辨識能力，可取代酵素和抗體，且具有高化學穩定性、便宜、容易製備，恰好可以克服感測器使用生物分子所面臨的限制和困難。目前分子拓印技術已被應用在分離技術的開發上，並已逐漸使用在生物感測器中，根據訊號傳導器的不同，可分成光學、電化學及重量偵測三大類，在本文中將會介紹分子拓印高分子的原理、分子拓印高分子型感測器的製作和應用，同時討論分子拓印高分子感測器未來的發展。

並列摘要

Biosensors have been used extensively in diagnostics, medicine, environmental monitoring and food processes. Various biological molecules such as antibodies and enzymes, which are capable of recognizing a specific target molecule, can be used as the biorecognition elements in biosensors. However, the cost and inactivation under harsh environments such as high pH and high temperature hamper the use of macrobiomolecules. In addition, it is difficult to find suitable enzymes or receptors that are able to recognize certain target analytes. Molecularly imprinted polymers (MIPs) are highly cross-linked synthetic polymers, which can be obtained from the innovative technique of molecular imprinting technique. MIPs have many advantages, including stability under hars environment, the simplicity of their preparation and can be used as alternatives for enzymes and antibodies. In recent years, MIPs have received much attention on applications to sensor design, the separation of drugs and chiral compounds and catalysis. In this text, the principles of MIPs preparation will be introduced. The MIP-based sensors can be classified as optical sensors, electrochemical sensors and mass-sensitive sensors. In addition, the fabrication and application of molecularly imprinted polymer-based sensors will be presented and discussed.