Chlorpheniramine對離體大白鼠氣管平滑肌的效應

Effects of Chlorpheniramine on Isolated Tracheal Smooth Muscle of Rat

王興萬(Hsing-Won Wang) ； 王愈婷(Yueh-Ting Wang) ； 吳其中(Chi-Chung Wu)

《台灣耳鼻喉頭頸外科雜誌》 45卷5期 (2010/10) Pp. 140-144

https://doi.org/10.6286/2010.45.5.140

摘要

目的：chlortrimeton(CTM)爲一傳統抗組織胺，屬於H1抗組織胺，可以減緩許多過敏性鼻炎的徵兆和症狀，特別是打噴嚏、鼻癢、流鼻水等。H1抗組織胺對鼻黏膜的作用，已眾所皆知，但對氣管平滑肌的作用，則有待探索。本研究旨在探討CTM對離體大白鼠氣管平滑肌的效應。
方法：本研究採用離體組織浴設置，以測試離體大白鼠氣管於不同濃度CTM的收縮效應。採用以下三種方法評估：(1)CTM對基本張力的影響；(2)CTM對離體氣管平滑肌因電場刺激引起收縮的效應；(3)CTM對離體氣管平滑肌因10^(-6)M methacholine引起收縮的效應。
結果：當氣管於張力穩定後，施予電刺激，氣管可隨電刺激強度的改變而有不同的收縮。CTM對氣管基本張力並無效應，但對10^(-6) methacholine所引起的氣管收縮有少許的舒張效應，10^(-6)M以上的CTM濃度可少許對抗methacholine所引起的收縮。CTM也可抑制氣管因電場刺激所引起的收縮。
結論：本研究設置簡便，結果有效、可靠，氣管的收縮或舒張會隨著藥物的劑量而有不同程度的改變。本研究顯示，大劑量的CTM可少許的減緩氣喘現象，CTM也有拮抗副交感神經反應的效果。

關鍵字

第一型抗組織胺 ； 氣管 ； 平滑肌 ； 離體測試

並列摘要

BACKGROUND: Chlotrimeton (CTM) is a classical antihistamine. It is often used as a classical histamine receptor-l (H1) antagonist in rhinitis patients who are suffering from sneezing and rhinorrhea. The effect of H1 antagonist on nasal mucosa in vivo is well known; however, the effect of the drug on tracheal smooth muscle has been rarely explored. The aims of this paper were to study the effects of CTM on isolated rat's tracheal smooth muscle.
MATERIALS AND METHODS: We used our preparation to test the effectiveness of CTM on isolated rat's tracheal smooth muscle. The following assessments of CTM were performed: (1) effect on tracheal smooth muscle resting tension; (2) effect on contraction caused by 10^(-6) M methacholine as a parasympathetic mimetic; (3) effect of the drug on electrically induced tracheal smooth muscle contractions.
RESULTS: Results indicated that addition of a parasympathetic mimetic to the incubation medium caused the trachea to contract in a dose-dependent manner. CTM had a minimal effect on the basal tension of trachea as the concentration increased. Addition of CTM at doses of 10^(-6) M or above elicited a relaxation response to 10^(-6) M methacholine-induced contraction. CTM could also inhibit electrical field stimulation (EFS) induced spike contraction.
CONCLUSION: This study indicated that high concentrations of CTM might actually antagonize the methacholine-induced contraction and could relief the symptoms of asthma. At the same concentrations of CTM, it might inhibit parasympathetic function of the trachea.

並列關鍵字

H1 antihistamine ； trachea ； smooth muscle ； in vitro study