A Survey of Vibrio parahaemolyticus in Imported Fishery Products in the Kaohsiung Area

M. C. Chen ； H. C. Huang ； M. H. Lee ； T. P. Liu ； C. T. Lee ； T. N. Wu

《Epidemiology Bulletin》 15卷5期 (1999/05) Pp. 77-86

https://doi.org/10.6525/TEB.199905_15(5).0001

並列摘要

The purpose of this study is to investigate the existence and distribution of Vibrio paraphaymolyticus, in the fishery products imported from different Asian countries. In addition, toxigenic genes related to Vibroi paraphaymolyticus, such as thermostable direct hemolysin (TDH) and thermostable direct related hemolysin (TRH) are tested. Between July 1997 and April 1998, specimens collected from 686 fishery products imported through the Kaohsiung International Airport were tested of Vibrio parahaymolyticus and its toxigenic genes, Vibro parahaemolyticus was isolated in 45.9% of the speciments, 81.3% in green crabs imported form Thailand, 75.8% in live shrimps imported from Thailand, and 71.1% in green crabs imported from Hong Kong. Products of different countries of origin showed statistically significant differences (p＜0.01). The results of statistical analyses indicated that the detection rate was higher in the crustaceans (49.4%) than in the non-crustaceans(37.4%). Detection rates by month ranged from 36.7% to 51.1%, showing little variation. Testing for toxigenic genes of Vibroi parahemolyticus such as TDH and TRH by month ranged from 36.7% to 51.1%, showing little variation. Testing for toxigenic genes of Vibrio parahemolyticus such as TDH and TRH by polymerase chain reaction(PCR) in fishery products gave negative responses in 187 product items. Though toxigenic genes in the present study were negative, reports are that Kanagawa-phenomenon negative strains can also generate cellular toxicity in animal experiments to cause deaths in rats and induce accumulation of ileum tract fluids in rabbits . The high detection rate of Vibroi parahaemolyticus in imported fishery producrs is a warning . Appropriate cooking and freezing of fishery products are most important methods in the prevention of Vibroi parahaemolyticus poisoning.