Sequential tunneling behavior in an InP/InGaAs p-n resonant tunneling diode with four-period superlattice is demonstrated. For the requirement of more negative differential resistance (NDR) routes, three split quantized energies are formed in the four-period InP/InGaAs superlattice structure with relatively thin InGaAs quantum wells under ideal flat-band condition, and high-field domain in the superlattice is formed under sufficiently large operation bias. Experimentally, an interesting six-route NDR characteristic, resulting from the form of split miniband structures and the extension of high-field domain in the superlattice, is observed at room temperature. Consequently, the studied device shows a good potential for multiple-valued logic circuit applications.