- 期刊
Expression of the Na-K-2Cl Cotransporter in Branchial Mitochondrion-Rich Cells of Mozambique Tilapia (Oreochromis mossambicus) Subjected to Varying Chloride Conditions
並列摘要
The Na/K/2Cl cotransporter (NKCC) plays a critical role of active Cl(superscript -) transport through the basolateral membranes of mitochondrion-rich (MR) cells in seawater teleosts. Recently, the NKCC was also found in apical membranes of MR cells of freshwater (FW)-acclimated tilapia (Oreochromis mossambicus). In this study, immunocytochemistry and immunoblotting were used to analyze the expression levels of the NKCC in FW-acclimated tilapia in response to different ambient chloride levels. The amount of branchial NKCC from low-Cl(superscript -)- (0.002-0.006 mM)-acclimated tilapia was about 3-and 5.5-fold higher than that from normal-(0.49-0.51 mM) and high-Cl(superscript -) (9.8-10.2 mM)-acclimated tilapia. With confocal microscopy, the NKCC was localized mainly in the apical membranes of MR cells with Cl(superscript -)-dependent abundances. The staining intensity of the NKCC in individual MR cells was significantly higher in the low-Cl(superscript -) group than in the normal and high-Cl(superscript -) groups. Results suggest that the apically localized NKCC is induced by a Cl(superscript -) deficiency and is probably also involved in the Cl(superscript -) uptake mechanism of MR cells.
延伸閱讀
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