Molasses contains high concentrations of caramelization pigment and sugars. This research studied the use of different types of cation exchange resins saturated with calcium ion, to separate the brown pigment and the sugars. The separation was achieved by utilizing different interactions between sugar molecules, pigment and immobilized calcium ion. Result shows that porous 50W-X8 resin demonstrated the best separation effect. Pretreatment involved acidification with sulfuric acid, 1:1 dilution with water followed by centrifugation. For a resin bed of 120 cm in length, 61% of the pigment was recovered and the recovery of sugar was 78%. Best separation conditions were pH4, 1 mL/min flow rate (column diameter was 1.8 cm) and a loading of 7.5 mL.