Analysis of Bioactive Triterpenes in Eriobotrya japonica LINDL. by High-Performance Liquid Chromatography

以高效液相層析法分析枇杷葉中具有活性之三萜類成分

何惠雅(Hui-Ya Ho) ； 林文川(Wen-Chuan Lin) ； 北中進(Susumu Kitanaka) ； 張淳堆(Chwen-Tzuei Chang) ； 吳金濱(Jin-Bin Wu)

《藥物食品分析》 16卷6期 (2008/12) Pp. 41-45

摘要

枇杷葉(Eriobotrya japonica)屬薔薇科(Rosaceae)具清肺止咳，和胃降逆的作用。本研究利用高效液相層析分析方法分析不同產地之枇杷葉中所含五種三萜類成分含量。樣品採用HyPURITY C-18(5 μm, 4.6×250 mm)管柱，移動相爲甲醇－0.15%醋酸水溶液（甲醇︰0.15%醋酸水溶液＝85:15, v/v）流速0.5 mL/min，三萜類成分以折射檢測器(RID)作分析得到良好分析結果。檢量線之回歸線性相關係數(r)爲0.9998-1.0000呈現良好線性關係。回收率在98.39%-102.41%之間顯示準確性佳。

關鍵字

枇杷 ； 高效液相層析 ； tormentic acid ； maslinic acid ； corosolic acid ； oleanolic acid ； ursolic acid

並列摘要

Eriobotrya japonica (Rosaceae) has been used for clearing away heat in the lung to stop cough and lowering the adverse flow of gi to stop vomiting. High-performance liquid chromatographic method was applied to assay the five triterpenes contents in E. japonica from various locations. Samples were analyzed on a HyPURITY C-18 column (5 μm, 4.6×250 mm) eluted at a rate of 0.5 mL/min with a solvent of A-B (A, Methanol; B, 0.15% acetic acid aqueous; A:B=85:15, v/v). Triterpenes were detected by refractive index detection (RID). Regression equations of the calibration curves revealed good linear relationships (correlation coefficients: 0.9998-1.0000). The recoveries ranged between 98.39%-102.41% and indicated good accuracy.

並列關鍵字

Eriobotrya japonica ； HPLC ； tormentic acid ； maslinic acid ； corosolic acid ； oleanolic acid ； ursolic acid