Simultaneous Quantification of Five Compounds from Nauclea officinalis Leaves by High Performance Liquid Chromatography

高液相色譜法同時測定瞻木葉中五個化合物的含量

謝達溫(Da-Wen Xie) ； 李永輝(Yong-Hui Li) ； 趙麗(Li Zhao) ； 丁崗(Gang Ding) ； 原少偉(Shao-Wei Yuan) ； 徐甲(Jia Xu) ； 蕭偉(Wei Xiao) ； 王振中(Zhen-Zhong Wang)

《藥物食品分析》 20卷2期 (2012/06) Pp. 489-494

https://doi.org/10.6227/jfda.2012200209

並列摘要

A simple, rapid and validated method was developed for the simultaneous determination of five characteristic components, including three alkaloids (pumiloside, 10 - hydroxy strictosamide, and strictosamide), and two flavonoids (rutin and kaempferol-3-O-rutinoside), in the Nauclea officinalis leaves. The chromatographic separation was achieved with a lichrospher C-18 analytical column by gradient elution with (A) 0.1% aqueous phosphoric acid and (B) acetonitrile as mobile phase, with a flow rate of 1.0 mL/min. The column temperature was set at 30℃. Detection was carried out on a photodiode array detector at 226 nm. The method was validated for system suitability, precision, accuracy and lincarity. The average recovery of the method was 98.00 - 100.44% and Iinearity (r) was＞0.9993. Furthermore, the method was successfully applied to the simultaneous determination of five analytes in 10 samples of N. officinalis leaves from different harvesting time of the herb. The results suggested that the method could be efficiently employed to evaluate the quality of N. officinalis leaves from various sources, which would benefit its quality control.

並列關鍵字

column liquid chromatography ； quality control ； Nauclea offivinalis leaves ； alkaloids ； flavonoids

參考文獻

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