Quality Analysis of Raw and Processed Schisandra Chinensis Fructus by Simultaneous Determination of Eleven Bioactive Lignans Using RP-HPLC Method

RP-HPLC法同時測定五味子生品與炮製品中11種木酚素

陸兔林(Tu-Lin Lu) ； 胡俊揚(Jun-Yang Hu) ； 毛春芹(Chun-Qin Mao) ； 吳楊(Yang Wu) ； 殷放宙(Fang-Zhou Yin) ； 蔡寶昌(Bao-Chang Cai)

《藥物食品分析》 20卷4期 (2012/12) Pp. 922-929+985

https://doi.org/10.6227/jfda.2012200422

摘要

建立RP-HPLC法同時測定五味子（Schisandra chinensis）中11種具生物活性之木酚素，包括schisandrin、gomisin J、schisandrol B、angeloylgomisin H、gomisin G、schisantherin A、schisantherin B、deoxyschisandrin、γ-schisandrin、schisandrin B及schisandrin C。層析柱為Elite ODS C18 (250 mm × 4.6 mm，5 μm)，移動相為乙腈－水。11種木酚素在以下濃度範圍內線性關係良好(r ≥ 0.9995)：25.02 - 150.12 μg/mL (schisandrin)；5.20 - 31.20 μg/mL (gomisin J)；10.99 - 65.94 μg/mL (schisandrol B)；14.10 - 84.60 μg/mL (angeloylgomisin H)；2.55 - 15.30 μg/mL (gomisin G)；3.79 - 22.74 μg/mL (schisantherin A)；8.32 - 49.92 μg/mL (schisantherin B)；5.16 - 30.96 μg/mL (deoxyschisandrin)；9.10 - 54.60 μg/mL (γ-schisandrin)；20.70 - 124.20 μg/mL (schisandrin B)；4.56 - 27.36 μg/mL (schisandrin C)。平均回收率為97.74 - 102.71%。本方法經精密性、再現性及準確性確效，應用於五味子生品及炮製品檢驗，可同時測定五味子中11種木酚素含量。結果顯示，醋五味子與酒五味子中11種木酚素含量較生品高，證實五味子生品與炮製品功效有別，各成分含量百分比為五味子品種挑選和臨床應用提供科學依據。

關鍵字

五味子 ； 木脂素 ； 炮製 ； HPLC ； 品質控制

並列摘要

A reversed-phase high-performance liquid chromatography (RP-HPLC) method was developed for the simultaneous determination of eleven bioactive lignans, namely schisandrin, gomisin J, schisandrol B, angeloylgomisin H, gomisin G, schisantherin A, schisantherin B, deoxyschisandrin, γ-schisandrin, schisandrin B and schisandrin C in Schisandra chinensis with UV detection. An Elite ODS C18 column (250 mm × 4.6 mm, 5 μm) was used for chromatographic separation. The mobile phase consisted of acetonitrile and water under gradient elution. All the calibration curves of the eleven bioactive lignans showed excellent linearity (r ≥ 0.9995) within the ranges of 25.02-150.1 μg/mL for schisandrin, 5.20-31.20 μg/mL for gomisin J, 10.99-65.94 μg/mL for schisandrol B, 14.10-84.60 μg/mL for angeloylgomisin H, 2.55-15.30 μg/mL for gomisin G, 3.79-22.74 μg/mL for schisantherin A, 8.32-49.92 μg/mL for schisantherin B, 5.16-30.96 μg/mL for deoxyschisandrin, 9.10-54.60 μg/mL for γ-schisandrin, 20.70-124.2 μg/mL for schisandrin B and 4.56-27.36 μg/mL for schisandrin C. The average recoveries ranged from 97.74 to 102.71%. This analytical method was also validated with respect to precision, repeatability and accuracy; and it was proven to be sensitive and accurate to simultaneously determine the eleven lignans in S. chinensis. The developed method was further applied to quantify the contents of the eleven lignans in raw and processed S. chinensis. The results revealed that the contents of the eleven lignans increased after processing with vinegar and wine. The lignans profiles obtained by this newly established method provided valuable information for the differentiation of crude and processed S. chinensis and the different effects. These content ratio differences could provide a scientific basis for the selection of origins and clinical usage.

並列關鍵字

Schisandra chinensis ； lignans ； processed ； high-performance liquid chromatography ； quality control

參考文獻

Fujihashi, T., Hara, H. and Sakata, T. et al. 1995. Anti-human immunodeficiency virus (HIV) activities of halogenated gomisin J derivatives, new nonnucleoside inhibitors of HIV type 1 reverse transcriptase. Antimicrobial Agents and Chemotherapy 39: 2000-2007.

Huang, M., Jin, J. and Sun, H. et al. 2008. Reversal of P-glycoprotein-mediated multidrug resistance of cancer cells by five schizandrins isolated from the Chinese herb Fructus Schizandrae. Cancer Chemotherapy and Pharmacology 62: 1015-1026.

Hancke, J. L., Burgos, R. A. and Ahumada, F. 1999. Schisandra chinensis (Turcz.) Baill. Fitoterapia70: 451-471.

Kim, S. R., Lee, M. K. and Koo, K. A. et al. 2004. Dibenzocyclooctadiene lignans from Schisandra chinensis protect primary cultures of rat cortical cells from glutamate-induced toxicity. Journal of Neuroscience Research 76: 397-405.

Huang, F., Xiong, Y. T. and Xu, L. H. et al. 2007. Sedative and hypnotic activities of the ethanol fraction from Fructus Schisandrae in mice and rats. J. Ethnopharmacol 110: 471-475.