茼蒿白化嵌紋病之病因診斷

The Diagnosis of Garland Chrysanthemum Albinic Mosaic Disease

陳煜焜(Y. K. Chen) ； 陳翠妙(T. M. Chen) ； 陳脉紀(M. J. Chen)

《植物病理學會刊》 5卷1期 (1996/03) Pp. 55-62

https://doi.org/10.6649/PPB.199603_5(1).0007

摘要

茼蒿白化嵌紋病毒（garland chrysanthemum albinic mosaic virus)，經分離、純化、測定病原性後，證實為引起茼蒿(Chrysanthemum coronariurn var. spatiosum)產生白化、嵌紋、裂葉等症狀之病原。病毒顆粒為長絲狀，平均大小約775×13 nm。機械接種感染油菜（Brasica campestris）、青江白菜（B. campestris ssp. chinensis)、白菜(B. campestris ssp. chinenisis var. communis)、蘿蔔 (Raphanus sativus）和菠菜（Spinacia oleracea)引起系統性嵌紋病徵：感染奎藜(Chenopodium quinoa)、紅藜(C. amaranticolor)、千日紅（Gomphrena globosa）和菸草（Nicotiana tabacum cv. Van-Hicks)，產生局部壞疽性病斑。罹病細胞內形成多數為板層狀聚合體之內含體，間雜少量捲軸狀者。病毒鞘蛋白分子量約37 kDa。瓊脂雙向擴散、酵素連結免疫吸附分析及免疫膠金標定等反應均顯示該病毒與蕪菁嵌紋病毒(turnip mosaic potyvirus. TuMV)有不可區分之血清類緣關係。本文證實茼蒿為TuMV之天然寄主，亦為茼蒿白化嵌紋病之首次報導。

關鍵字

茼蒿 ； 蕪菁嵌紋病毒

並列摘要

A virus, designated as garland chrysanthemum albinic mosaic virus (GCAMV), causing albinism, mosaic, leaf-cleavage and stunting in garland chrysanthemum (Chrysanthemum coronarium var. spatiosum Bailey) was isolated and purified. The virus was transmissible by mechanical means. Upon artificial inoculation, it caused mosaic symptoms on garland chrysanthemum, rape (Brassica campestris), Chinese mustard (B. campestris ssp. chinensis), Chinese white cabbage (B. campestris ssp. chinese var. communis) and spinach (Spinacia oleracea), and necrotic local lesions on Chenopodium quinoa, C. amaranticolor. Gomphrena globosa and Nicotiana tabacum cv. Van Hicks. Electron microscopy of diluted crude sap and purified virus preparations revealed numerous filamentous particles with an average size of approximately 775 x 13 nm. Cytoplasmic inclusions of laminated aggregates and scrolls were observed in the cytoplasm of systemically infected test plants. Data obtained from SDS-immunodiffusion, ELISA and gold-labelled antibody decoration (GLAD) further indicated that GCAMV was indistinguishable from, and was serologically related to turnip mosaic virus (TuMV). This is the first report of garland chrysanthemum as a natural host of TuMV.

並列關鍵字

Chrysanthemum coronarium var. spatiosum ； garland chrysanthemum ； turnip mosaic virus