Transposition and aggressiveness in a Phaeosphaeria nodorum sexual Cross

小麥葉枯病菌(Phaeasphaeria nodorum)雜交子代的基因轉位與致病毒力分析

翁溥(P.P.Ueng) ； E. Arseniuk ； B. M. Cunfer ； Q. J. Song

《植物病理學會刊》 12卷3期 (2003/09) Pp. 149-156

https://doi.org/10.6649/PPB.200309_12(3).0002

摘要

本研究利用致病毒力(aggressi veness)的遺傳分離、未命名的分子標記(anonymous molecular markers)及轉位子(transposon)等核酸探針分析小麥葉枯病菌(Phαeosphaeria nodorum)的雜交子代。評估的主要對象是對小麥具高致病力的葉枯病菌Sn26-1菌株與來自裸麥的Phaeosphaeria sp. Sn26-1菌株交配後的子代。此二親本菌株及其雜交子代對不同穀類的致病力強弱依序為:黑小麥〉小麥〉棵麥。本研究發現各子代間的致病力有不相同程度的差異。子代64-3興64-5菌株的致病力相等於或強於其二親本菌株，然而子代64-1及64-4菌株則因在培養中產孢量低或不產孢而致病力低或無致病力。利用未命的分子標記及轉記子等核酸探針分析所得之RELP，結果顯示子代菌株中共有六種指紋圖譜，且其分離現象係發生在兩次減數分裂形成子囊孢子的過程在在致病力及產孢量低的子代菌株中也出現數個非對稱的轉位子片段，文中也將討論轉位子片段與病理特性之可能關係。

關鍵字

小麥 ； 小麥葉枯病菌 ； 分子標記 ； 致病力；限制片段長度多型性(RFLP) ； 黑小麥 ； 裸麥 ； 轉位子

並列摘要

Genetic segregation of aggressiveness, anonymous molecular markers and transposon DNA probes in a . Phaeosphαeria nodorum sexual cross was studied. The progeny of a P. nodorum isolate with high aggressiveness to wheat (Sn26-1) and aPhaeosphaeria sp. from rye (Sn48-1) wasevaluated. The magnitude of aggressiveness to cereals caused by two cross parents and their progeny was triticale＞ wheat＞ rye. Different levels of aggressiveness were detected in the progeny. Progeny 64-3 and 64-5 had equal or higher aggressiveness than two parental isolates. Low or no aggressiveness in the progeny 64-1, 64-2, and 64-4 was due to low or no sporulation in these cultures. Using RFLP fingerprinting with anonymous molecular markers due to low or no sporulation in these cultures. Using RFLP fingerprinting with anonymous molecular markers and transposon DNA probes, six segregation patteerns were found in the progeny, and the segregation occurred in both meiotic divisions during ascospore formation. Several asymmetric transposontagged bands were detected in the progeny with low aggressiveness and low sporulation. The possible association of transposon-tagged bands with pathological characters is discussed.

並列關鍵字

aggressiveness ； molecular markers ； Phaeosphaeria spp. ； RFLP ； rye ； Stagonospora nodorum ； transposon ； triticale ； Triticum aestivum L. ； wheat