研製百合萎凋病菌的鑑別培養基

Development of a Selective Medium for Detecting Fusarium oxysporum f. sp. lilii

黃振文(J. W. Huang) ； 許嘉蘋(C. P. Shu) ； 陳志弘(C. H. Chen)

《植物病理學會刊》 14卷2期 (2005/06) Pp. 103-114

https://doi.org/10.6649/PPB.200506_14(2).0003

摘要

本文主要的目的於嚐試研發百合萎凋病菌(Fusarium oxysporum f. sp. lilii)的選擇性培養基，以利偵測百合萎凋病菌在種球與土壤中的存活。選擇適當的基礎培養基加入多種微生物抑制劑與調整酸鹼值後，測試它們對百合萎凋病菌生長的影響，結果顯示Komada培養基加入50ppm(ai)免賴得可濕性粉劑，並以10%(v/v)磷酸調整酸鹼值爲pH4.0後，所製成之FLI培養基，可以有效自百合種球與土壤中偵測出百合萎凋病菌。FLI培養基可抑制F. oxysporum f. sp. lactucae（萵苣萎凋病菌）、F. oxysporum f. sp. niveum（西瓜蔓割病菌）、F. oxysporum f. sp. momordicae（苦瓜萎凋病菌）、F. oxysporum f. sp. raphani（蘿蔔黃葉病）、F. oxysporum f. sp. cubense（香蕉黃葉病）、F. oxysporum f. sp. lycopersici（番茄萎凋病）等病原菌。此外亦可抑制F. avenaceum、F. camptoceras、f. concolor、F. dimerum、F. graminearum、F. subglutinans、F. semitectum、F. graminum、F. reticulatum、F. proliferatum、F. moniliforme、F. merismoides、F. lateritium及F. heterosporum等；並可由菌落形態與顔色將F. oxysporum f. sp. lilii與F. poae、F. solani、F. ventricosum區分開。進一步，利用FLI培養基自百合栽培田土與病株分離所得之F. oxysporum菌株接種於百合鱗片球後，證明在FLI培養基可生長之F. oxysporum菌株對百合植株均具有病原性。

關鍵字

百合萎凋病菌 ； 鐮胞菌 ； 百合 ； 偵測 ； 選擇性培養基

並列摘要

The FLI selective medium was successfully developed for detection of F. oxysporum f. sp. lilii from infested soil and diseased lily plants. The FLI selective medium (pH 4) consisted of 1.0g K2HPO4, 0.5g KCl, 0.5g MgSO4, 0.01g Fe-Na-EDTA. 2.0g L-asparagine. 20.0g Galactose, 1.0g pentachloronitrohenzene. 0.5g oxgall. 1.0g Na2B4O7, 10H2O, 0.3g streptomycin sulfate, 0.1 g benomyl(50%). 15.0g agar and 1.0L distilled water was more sensitive than Nash-PCNB medium for distinguishing the pathogen from the other Fusarium species. In the study, it was proved that F. oxysporum f. sp. lactucae. F. oxysporum f. sp. niveum. F. oxysporum f. sp. momordicae. F. oxysporum f. sp. raphani. F. oxysporum f. sp. cubense and F. oxysporum f. sp. lycopersici were not able to grow on the FLI medium plate. Among 29 species of Fusarium tested, except for F. poae, F. solani and F. ventricosum, the others could not grow on the FLI medium. Although F. poae, F. solani and F. ventricosum were able to grow on the FLI medium, their colony morphologies and color were completely different from ones of F. oxysporum f. sp. lilii. Once the isolates of F. oxysporum obtained from diseased lily plant parts and the infested soils were able to grow onto the FLI medium, they were certainly to show the pathogenicity to lily plants.

並列關鍵字

Detection ； Fusarium spp. ； F. oxysporum f. sp. lilii. ； selective medium