Effects of Cranberry Juice and Pineapple Juice on Pratylenchus coffeae

蔓越莓汁與鳳梨汁對Pratylenchus coffeae之殺線蟲效果

蔡碧雲(Bie-Yun Tsai)

《植物病理學會刊》 17卷2期 (2008/06) Pp. 111-118

https://doi.org/10.6649/PPB.200806_17(2).0002

摘要

蔓越莓汁殺死之線蟲，其表皮常可見類似人類皮膚燙傷時之起泡現象。用蔓越莓汁處理96小時後75.7% P. coffeae表皮有起泡現象。果糖或鳳梨汁處理之線蟲表皮皆無起泡。蔓越莓汁對不同齡期根腐線蟲皆有良好殺線蟲效果。處理四天後，二齡幼蟲及雄蟲致死率高達100%，雌成蟲致死率爲97%，三齡及四齡幼蟲致死率爲99%。蔓越莓汁稀釋5倍已失去殺線蟲效果，但仍有麻痺作用。處理96小時後有71.5%之根腐線蟲被麻痺。煮沸兩分鐘對蔓越莓汁之殺線蟲效果沒有影響。罐裝鳳梨汁對根腐線蟲完全沒有殺線蟲效果。手榨新鮮鳳梨汁則對根腐線蟲有良好之殺線蟲效果。煮沸兩分鐘會使手榨新鮮鳳梨汁完全喪失殺線蟲效果。對熱穩定性之差異及對線蟲表皮造成起泡能力之差異顯示蔓越莓汁及新鮮鳳梨汁之殺線蟲成分爲不同物質。於生長箱環境下，蔓越莓汁及新鮮鳳梨汁施用於沙土中皆可顯著降低根腐線蟲侵入綠豆苗根部。

關鍵字

蔓越莓 ； 鳳梨 ； Pratylenchus ； 防治

並列摘要

The nematodes killed by cranberry juice often had puffed cuticle similar to the blisters on human skin after been scalded by boiling water. There were 75.7% coffeae with puffed cuticles after 96 h exposure to cranberry juice. None of the nematodes in the fructose or pineapple juice treatment had puffed cuticle. Cranberry juice was very effective in killing all the developmental stages of P. coffeae. The mortality rates were 100% for J2 and male, 97% for female and 99% for J3+J4 after 96 h exposure. Dilution of cranberry juice to 1:5 rendered it ineffective in killing P. coffeae but 71.5% of nematodes were paralyzed after 96 h exposure. Cranberry juice remained very effective in killing P. coffeae after boiling for 2 minutes. The canned pineapple juice was completely ineffective in killing P. coffeae. However, hand-squeezed fresh pineapple juice caused high mortality of the nematodes. Boiling for 2 min rendered it completely ineffective. The cranberry juice and pineapple juice have different nematicidal principles as indicated by heat stability test and the difference in their ability to cause puffed cuticle. The infection of mung bean roots by P. coffeae was significantly reduced by cranberry juice and fresh pineapple juice in the growth chamber tests.

並列關鍵字

control ； cranberry ； juice ； pineapple ； Pratylenchus