伯利恆之星軟腐病之發生

許秀惠(S. H. Hseu) ； 宋嘉仁(C. J. Sung) ； 林俊義(C.Y. Lin)

《植物病理學會刊》 17卷2期 (2008/06) Pp. 177-182

https://doi.org/10.6649/PPB.200806_17(2).0008

摘要

於台中后里、嘉義新港、彰化田尾等地區栽培之伯利恆之星植株上發現組織水浸狀褐化軟腐的病徵，病徵通常從藥片基部或鱗莖開始發生，初呈水浸狀斑，迅速蔓延形成淡褐色或褐色或燃褐色軟腐，終至藥片或植株倒伏。經柯霍氏法則、生理生化、Biolog及PCR鑑定結果顯示分離所得之病原細菌包括Pectobacterium（原屬於Erwinia）carotovora subsp. carotovora (Ecc)及Pectobacterium（原屬於Erwinia）chrysanthemi (Ech)。調查田間鱗莖及葉片組織軟腐病徵發生情形，發現不論鱗莖或葉片均以最外層（一～三層）組織最常發生軟腐病徵，其次爲最內層組織，以Ec3F/Ec4R引子對檢測結果相同。以Ec3F/4R引子對檢測不同時期田間罹病植株的鱗莖及葉片組織，結果顯示不論鱗莖或葉片組織於五月、六月及十二月份所取樣品均可檢測到Ecc及Ech二種羅腐病原，於二月，三月及四月所取樣品檢測結果均只檢測到Ecc一種軟腐病原，未測到Ech。

關鍵字

伯利恒之星 ； 細菌性軟腐病 ； PCR ； 檢測

並列摘要

A soft-rot disease seas found on the star of Bethleham plants cultivated in Taichung Howli, Chiayi Singang, Chunghua Tianwei areas. Symptoms were usually seen at the base of leaves or bulbs, beginning with water soaking spots that developed into light brown or black brown soft-rot areas in late stage of infection. The diseased leaves or plants eventually fell down. Based on the physiological characteristics and the results from the Biolog GN MicroPlate system, polymerase chain reaction (PCR) and pathogenicity tests, bacteria isolated from the diseased tissues were identified as Pectobacerium (Erwinia) carotovora subsp. carotovora (Ecc) and Pectobacterium (Erwinia) chrysanthemi (Ech). Investigating the occurrence of soft-rot symptoms and PCR-detection using Ec3F/Ec4R primers on bulbs and leaf tissues in the fields showed that the outer layer tissue (layer l~3) has the highest disease incidence, followed by the inner tissue. PCR detection with Ec3F/Ec4R primers of infected tissues at different time in the fields resealed that both Ecc and Ech could be detected in May, June, and December on bulbs and leaf tissues, whereas only Ecc, but not Ech, could be detected in the samples collected in February, March and April.

並列關鍵字

Star of Bethleham ； bacterial soft rot disease ； PCR ； detection