轉基因策略發展雙生病毒抗性植物之現況及展望

Transgenic Strategies for Developing Transgenic Plants with Geminivirus Resistance

林靜宜(C.-Y. Lin) ； 蔡文錫(W.-S. Tsai) ； 古新梅(H.-M. Ku) ； 詹富智(F.-J. Jan)

《植物病理學會刊》 18卷4期 (2009/12) Pp. 185-200

https://doi.org/10.6649/PPB.200912_18(4).0001

摘要

雙生病毒(geminivirus)爲全球性的重要植物性病毒病原，可危害多種重要經濟及糧食作物，造成重大之經濟損失，嚴重影響農民收入與糧食供應。因此發展有效防治此病毒病害的策略有其重要性及應用價值。由於此病毒之基因體結構由單股DNA (ssDNA)組成，且易發生病毒之間的重組而具高度變異性，因此一般使用之傳統防治方法效果不盡理想且不易獲得高度抗性之植物。因此利用遺傳工程技術爲理論基礎，發展出有效的轉基因抗性，提供了另一有效的病毒防治途徑。本文針對目前利用轉基因策略發展雙生病毒抗性的相關研究作完整之彙整，包括藉由表現病毒蛋白、非病毒蛋白、基因沉寂與DNA干擾等策略來促使轉基因植物表現抗性等，以期能了解有效率的轉基因抗病模式，作爲研發持久且具廣泛性抗性轉基因植物的基石。

關鍵字

雙生病毒 ； 轉基因植物 ； 病毒抗性

並列摘要

Geminiviruses are serious threat to agriculture practice worldwide. The diseases cause high yield losses, especially in the economical crops, generating serious reduction of fanners' income and food supply in general. In order to solve these economic and social impacts, various strategies have been developed for virus control. Due to their s ingle-stranded DNA genomes and high diversity caused by frequently recombinalion intra-and inter-virus species, many traditional approaches have limited efficiency for virus control. Therefore, transgenic approach based on genetic engineering technology provides other efficient strategies. This article reviewed the recent studies related to transgenic resistance of geminivirus by different strategies, such as expression of viral of non-viral proteins, gene silencing and DNA interference. The implementation of these strategies will help us to comprehend the resistant model and also contributed to develop the transgenic plants with broad spectrum and durable resistance.

並列關鍵字

geminivirus ； transgenic plant ； viral resistance